Rabbit Anti-Phospho-MYC (T58+S62) Recombinant Antibody (clone 4E9) (CAT#: ZG-0478U)

Name: Rabbit Anti-Phospho-MYC (T58+S62) Recombinant Antibody (clone 4E9)
SKU: ZG-0478U
Rating: 5 (1 reviews)

Rabbit Anti-Phospho-MYC (T58+S62) Antibody (clone 4E9) displayed high specificity against the MYC protein of Human.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-Phospho-MYC (T58+S62) Antibody (ZG-0478U) in WB

Figure 1 Rabbit Anti-Phospho-MYC (T58+S62) Antibody (ZG-0478U) in WB

Western Blot
Positive WB detected in HepG2 whole cell lysate(treated with EGF or not)
All lanes Phospho-MYC antibody at 0.99μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 KDa
Observed band size: 57 KDa

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/if_selected.jpg

Figure 2 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66556/1279_E11_3_selected.jpg

Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/ihc_selected.jpg

Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/84/ihc_selected.jpg

Figure 5 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows distinct nucleolar positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/ihc_selected.jpg

Figure 6 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/883_H10_1_red_green.jpg

Figure 7 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/879_G9_1_red_green.jpg

Normal Tissue

Figure 8 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_7_5.jpg

Figure 9 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_4.jpg

Figure 10 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_7_5.jpg

Figure 11 Cervix
(Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_8_3.jpg

Figure 12 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_8.jpg

RNA Expression

Figure 13 RNA cell line category: Cell line enhanced (RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136997-MYC

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthesized peptide derived from human Phospho-MYC (T58+S62)

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human Phospho-MYC (T58+S62)

Species Reactivity
Human

Clone
4E9

Applications
ELISA, WB

Conjugate
Unconjugated

Related Disease
Burkitt Lymphoma and High-Grade B-Cell Lymphoma Double-Hit/Triple-Hit.

Product Property

Purification
Affinity chromatography purified

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000

Target

Alternative Names
Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39

Gene ID
4609

UniProt ID
P01106

Related Resources

Related Signaling Pathways
Related Diseases

BCR Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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