Rabbit Anti-Phospho-TP53 (T55) Recombinant Antibody (clone 3C3) (CAT#: ZG-0599U)
Rabbit Anti-Phospho-TP53 (T55) Antibody (clone 3C3) displayed high specificity against the TP53 protein of Human.
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Custom Production
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WB
Figure 1 Rabbit Anti-Phospho-TP53 (T55) Antibody (ZG-0599U) in WB
Western Blot
Positive WB detected in 293 whole cell lysate(treated with Calyculin A or Pervanadate)
All lanes Phospho-TP53 antibody at 1.28μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 KDa
Observed band size: 53 KDa
IF
Figure 2 Rabbit Anti-Phospho-TP53 (T55) Antibody (ZG-0599U) in IF
Immunofluorescence staining of 293 cells(treated with 50mM Calyculin A for 30min) with ZG-0599U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-TP53 (T55)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-TP53 (T55)
- Species Reactivity
- Human
- Clone
- 3C3
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Li-Fraumeni Syndrome and Papilloma Of Choroid Plexus.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53
- Gene ID
- 7157
- UniProt ID
- P04637
Related Resources
EGFR Tyrosine Kinase Inhibitor Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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|---|---|---|---|
| DrMAB-994 | Recombinant anti-TP53 antibody svFv Fragment | ELISA, Agonist | scFv |
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| HPAB-M0287-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 6C4); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0662-YJ-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone Ab421); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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