Mouse Anti-CD59 Recombinant Antibody (clone 8D2B8) (CAT#: VS3-XY382)
Clone 8D2B8 is a mouse antibody, which reacts with human CD59 protein. This antibody is suitable for CD59 detection by ELISA, WB, IHC, FC, ICC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/279_B8_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/279_B8_2_selected.jpg
Figure 2 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows distinct membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows distinct membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/ihc_selected.jpg
Figure 3 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to the vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/1432_F1_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to the vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/1432_F1_2_blue_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/280_B8_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/280_B8_1_blue_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55053_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55053_B_8_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55159_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55159_A_7_3.jpg
Figure 7 Urinary bladder
(Urothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55159_A_6_5.jpg
(Urothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55159_A_6_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (TIME, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085063-CD59
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085063-CD59
Figure 8 RNA cell line category: Cell line enhanced (TIME, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085063-CD59
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085063-CD59
Specifications
- Immunogen
- Recombinant fragment of human CD59 (a.a. 31-111) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CD59
- Species Reactivity
- Human
- Clone
- 8D2B8
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
- Gene ID
- 966
- UniProt ID
- P13987
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1088z | Mouse Anti-CD59 Recombinant Antibody (clone 18C6) | FC, IF, FuncS | Mouse IgM, κ |
| FAMAB-1074CQ | Mouse Anti-CD59 Recombinant Antibody (clone YTH 53.1) | FC | Mouse IgG1 |
| ZG-0208U | Mouse Anti-CD59 Recombinant Antibody (clone 2E11B6) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-WK768 | Mouse Anti-CD59 Recombinant Antibody (clone OV9A2) | FC, IF | Mouse IgG1 |
| VS11-QX41 | Mouse Anti-CD59 Recombinant Antibody (clone 193-27) | FC, IHC, IF | Mouse IgM, κ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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