Human Anti-CD59 (clone 1E8) scFv-Fc Chimera
CAT#: VS-0125-FY58
The recombinant 1E8 scFv-Fc is a single-chain antibody with specificity for CD59. Its design allows for the linkage of both the heavy and light chains of the antibody on the same chain, along with the binding of the human Fc region (CH2-CH3) to enhance its stability and functionality. The recombinant 1E8 scFv-Fc antibody was expressed and secreted into the culture medium after transfection of 293T cells. Flow cytometry analysis revealed that CD59 was highly expressed in prostate cancer cells, and the recombinant 1E8 scFv-Fc exhibited significantly enhanced complement-dependent cytotoxicity (CDC) similar to that of the mouse 1E8 antibody.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/279_B8_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows distinct membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to the vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/1432_F1_2_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/280_B8_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55053_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55159_A_7_3.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26494/55159_A_6_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (TIME, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085063-CD59
❮
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- clone 1E8
- Applications
- ELISA, Flow Cytometry, Complement-dependent cytolysis
- Related Disease
- Prostate cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The anti-CD59 single-chain antibody (scFv-Fc), known as 1E8 scFv-Fc, has the potential to serve as an effective adjuvant therapy in cancer treatment. By targeting CD59-expressing cancer cells, this recombinant antibody may enhance complement-dependent cytotoxicity (CDC), leading to increased tumor cell lysis and death. Given that CD59 is a key regulator of the complement system that inhibits complement-mediated cell death, the binding of 1E8 scFv-Fc to CD59 on the surface of tumor cells could activate the complement cascade. This activation may bolster the anti-tumor immune response, offering a promising avenue for improving cancer therapies.
Target
- Alternative Names
- CD59 Molecule (CD59 Blood Group); CD59 Antigen P18-20 (Antigen Identified By Monoclonal Antibodies 16.3A5, EJ16, EJ30, EL32 And G344); CD59 Molecule, Complement Regulatory Protein; Membrane Attack Complex Inhibition Factor; Membrane Inhibitor Of Reactive Lysis; 20 KDa Homologous Restriction Factor; MEM43 Antigen; 1F5 Antigen; Protectin; HRF-20; MAC-IP; MIC11; MSK21; HRF20; MACIF; MIN1; MIN2; MIN3; MIRL; Surface Anitgen Recognized By Monoclonal Antibody 16.3A5
- Gene ID
- 966
- UniProt ID
- P13987
- Cellular Localization
- Cell membrane; Lipid-anchor, GPI-anchor
Secreted
- Post Translation Modifications
- N- and O-glycosylated.
Glycated.
Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects.
Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
Glycosylation at Thr40, Asn43, Lys66, Thr76, Thr77, Thr105, Ser106, and Thr111
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect CD59_HUMAN (79)
Glycosylation from GlyGen 7 sites, 216 N-linked glycans (2 sites), 9 O-linked glycans (6 sites)
- Protein Refseq
- NP_000602.1; NP_001120695.1; NP_001120697.1; NP_001120698.1; NP_001120699.1
- Function
- Potent inhibitor of the complement membrane attack complex (MAC) action.
Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore.
This inhibitor appears to be species-specific.
Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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