What protein does this antibody recognize?

This antibody recognizes MMP9 (Matrix Metallopeptidase 9), an enzyme involved in the breakdown of extracellular matrix. It recognizes the protein in Human and Mouse samples.

Is this antibody validated for Flow Cytometry?

Yes, this Rabbit Polyclonal Antibody can be used for Flow Cytometry (FC), in addition to Western Blot (WB), Immunofluorescence (IF), and Immunohistochemistry (IHC).

Rabbit Anti-MMP9 Polyclonal Antibody (MRO-2039-CN)

CAT#: MRO-2039-CN

This antibody is a rabbit polyclonal antibody that is capable of binding to MMP9.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg

Subcellular Location Figure 2 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg

Normal Tissue Figure 5 Testis

Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enriched (NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9

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Specifications

Immunogen
Peptide

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human MMP9 (Matrix Metallopeptidase 9)

Species Reactivity
Human, Mouse

Applications
Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Flow Cytometry

Conjugate
Unconjugated

Product Property

Purification
Peptide affinity purified

Format
Liquid

Buffer
1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol

Preservative
0.05% Sodium Azide

Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

Alternative Names
Matrix Metallopeptidase 9; Matrix Metalloproteinase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase); EC 3.4.24.35; CLG4B; MMP-9; GELB; Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase); Matrix Metalloproteinase-9;

Gene ID
4318

UniProt ID
P14780

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Breast cancer biomarkers at key points during disease progression

Strong Western Blot Signal

We utilized this antibody to detect MMP9 in cell culture supernatants via Western Blot. It successfully identified the protein with strong signal intensity. It is a versatile antibody that also works well for Flow Cytometry and Immunofluorescence in our hands.

Effective for Tissue Remodeling

We study tissue remodeling, and this anti-MMP9 antibody is a key reagent for us. We used it in IHC to visualize MMP9 expression in tissues. The staining was clear and specific. The broad reactivity with human and mouse makes it excellent for translational research.

Q&As

What protein does this antibody recognize?

A: This antibody recognizes MMP9 (Matrix Metallopeptidase 9), an enzyme involved in the breakdown of extracellular matrix. It recognizes the protein in Human and Mouse samples.
Is this antibody validated for Flow Cytometry?

A: Yes, this Rabbit Polyclonal Antibody can be used for Flow Cytometry (FC), in addition to Western Blot (WB), Immunofluorescence (IF), and Immunohistochemistry (IHC).

View the frequently asked questions answered by Creative Biolabs Support.

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Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MRO-2039-CN.pdf

MSDS

MSDS - MRO-2039-CN.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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