Rabbit Anti-Smad2 (phospho S250) Antibody
CAT#: VS-0126-XL1601
Specifically identifies Smad2 modified at Serine 250. This rabbit-derived polyclonal antibody recognizes MAPK-mediated linker-region regulation; optimized specifically for performance in Western Blot (WB).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
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Specifications
- Host Species
- Rabbit
- Specificity
- SMAD2
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Specifically detecting Smad2 phosphorylated at Ser250, this rabbit polyclonal antibody monitors linker-site regulation. It is a precise probe for exploring crosstalk between MAPK and TGF-beta signaling pathways in cells.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:1000
Target
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Related Diseases
Colorectal Cancer
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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