Recombinant Rabbit Anti-B2M Antibody (clone R01-4H5) (CAT#: VS3-FY128)

This product is a recombinant rabbit antibody that recognizes human and mouse B2M protein. It can detect a band of approximately 14 kDa (observedd molecular weight: 14 kDa).


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WB

Figure 1. Recombinant Rabbit Anti-B2M Antibody (clone R01-4H5) in WB.

Figure 1. Recombinant Rabbit Anti-B2M Antibody (clone R01-4H5) in WB.

Western Blot Analysis of β2 Microglobulin in HeLa Lysates Using β2 Microglobulin.

IHC

Figure 2. Recombinant Rabbit Anti-B2M Antibody (clone R01-4H5) in IHC.

Figure 2. Recombinant Rabbit Anti-B2M Antibody (clone R01-4H5) in IHC.

Immunohistochemical analysis of paraffin-embedded mouse colon using beta 2.


Specifications

  • Immunogen
  • Recombinant protein of human beta 2 Microglobulin
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Mouse B2M
  • Species Reactivity
  • Human, Mouse
  • Clone
  • R01-4H5
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 14 kDa; Observed MW: 14 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95%
  • Format
  • Liquid
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Beta-2-Microglobulin; Beta Chain Of MHC Class I Molecules; Beta-2-Microglobin; IMD43
  • Sequence Similarities
  • Belongs to the beta-2-microglobulin family.
  • Cellular Localization
  • Amyloid, MHC I, Secreted
  • Post Translation Modifications
  • Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Function
  • Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "B2M"

Single-domain Antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-1010MZ Chicken Anti-beta-2 Microglobulin Polyclonal IgY WB Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-034CQ Mouse Anti-B2M Recombinant Antibody (clone YTH470.5) FC, Block Mouse IgG1, κ

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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