Recombinant Rabbit Anti-F3 Antibody (clone R04-7G2)

Name: Recombinant Rabbit Anti-F3 Antibody (clone R04-7G2)
SKU: VS3-FY530
Rating: 5 (1 reviews)

CAT#: VS3-FY530

This product is a recombinant rabbit antibody that recognizes F3. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R04-7G2 is specific for human, mouse, rat F3. The antigen is human tissue factor synthetic peptides.

Gene Expression Tested Data

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49292/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line HaCaT

Immunofluorescent staining of human cell line HaCaT shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69132/1343_C8_1_selected.jpg

Subcellular Location Figure 3 IHC staining of human nasopharynx

Immunohistochemical staining of human nasopharynx shows strong cytoplasmic positivity in respiratory epithelial cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/ihc_selected.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_B_7_5.jpg

Normal Tissue Figure 5 Testis

Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_A_6_6.jpg

Normal Tissue Figure 6 Placenta

Decidual cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_A_2_7.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (A-431, HaCaT, HBEC3-KT, U-2197)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117525-F3

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IHC-P

Figure 1. Recombinant Rabbit Anti-F3 Antibody (clone R04-7G2) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using tissue factor antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

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Specifications

Immunogen
Human Tissue Factor Synthetic Peptides.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat F3

Species Reactivity
Human, Mouse, Rat

Clone
R04-7G2

Applications
Western Blot, Immunohistochemistry-Paraffin

Conjugate
Unconjugated

MW
Calculated MW: 33 kDa; Observed MW: 45 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100

Target

Alternative Names
Coagulation Factor III, Tissue Factor; Coagulation Factor III (Thromboplastin, Tissue Factor); Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142 Antigen; CD142; TFA

Gene ID
2152

UniProt ID
P13726

Sequence Similarities
Belongs to the tissue factor family.

Cellular Localization
Membrane, Secreted

Protein Refseq
NP_001171567.1; NP_001984.1

Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-FY530.pdf

MSDS

MSDS - VS3-FY530.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Secondary Antibody

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