Mouse Anti-APP Recombinant Antibody (clone 10D5) (CAT#: PABL-401)
Recombinant Mouse Antibody (10D5) is capable of binding to APP. This antibody recognizes Aβ residues 3–7.
Specific Inquiry
IF
Figure 1 Application of anti-amyloid antibody 10D5 clears amyloid-β plaques.
Amyloid-β plaque clearance occurred only in cortical tissue treated with 10D5. Amyloid protein was immunostained with fluorescent 3D6. Untreated area of cortexin animal treated with 10D5. Plaques are clearly visible (arrow). Scale bar, 50μm.
Lombardo, J. A., Stern, E. A., McLellan, M. E., Kajdasz, S. T., Hickey, G. A., Bacskai, B. J., & Hyman, B. T. (2003). Amyloid-β antibody treatment leads to rapid normalization of plaque-induced neuritic alterations. Journal of Neuroscience, 23(34), 10879-10883.
FuncS
Figure 2 Application of anti-amyloid antibody 10D5 clears amyloid-β plaques.
Comparison of amyloid burden measured with 3D6 antibody under different experimental conditions. The values are the means; error bars indicate SD.
Lombardo, J. A., Stern, E. A., McLellan, M. E., Kajdasz, S. T., Hickey, G. A., Bacskai, B. J., & Hyman, B. T. (2003). Amyloid-β antibody treatment leads to rapid normalization of plaque-induced neuritic alterations. Journal of Neuroscience, 23(34), 10879-10883.
IF
Figure 3 Amyloid-β clearance restores the morphology of neurites.
Untreated area of cortex from 10D5-treated animal, immunostained with Cy-3-SMI-32.
Lombardo, J. A., Stern, E. A., McLellan, M. E., Kajdasz, S. T., Hickey, G. A., Bacskai, B. J., & Hyman, B. T. (2003). Amyloid-β antibody treatment leads to rapid normalization of plaque-induced neuritic alterations. Journal of Neuroscience, 23(34), 10879-10883.
Specifications
- Immunogen
- Human amyloid beta (A4) precursor protein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Specificity
- APP
- Species Reactivity
- Human
- Clone
- 10D5
- Applications
- IF, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence and Function Assay.
Target
- Alternative Names
- APP; amyloid beta (A4) precursor protein; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma; amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); alzheimer disease amyloid protein; cerebral vascular amyloid peptide
- Gene ID
- 351
- UniProt ID
- P05067
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-228 | Anti-Human Abeta Recombinant Antibody (Solanezumab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
| TAB-227 | Anti-Human Abeta Recombinant Antibody (Ponezumab) | WB, ELISA, IP, FC, FuncS, Neut, IF | IgG2 - kappa |
| TAB-253CQ-F(E) | Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-253CQ-F(E)) | ELISA | Humanized Fab |
| TAB-0790CLV-F(E) | Mouse Anti-APP Recombinant Antibody; Fab Fragment (TAB-0790CLV-F(E)) | ELISA | Humanized Fab |
| TAB-0828CLV-F(E) | Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0828CLV-F(E)) | ELISA | Humanized Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Extremely Specific
The Mouse Anti-APP Recombinant Antibody (clone 10D5) exhibits outstanding specificity in our Western blot experiments. It detects APP without cross-reacting with other proteins, making it indispensable in our research.
Well Cited
The fact that this antibody is widely cited in peer-reviewed literature gave us confidence in its utility. It has lived up to the reputation, providing robust results for our APP studies.
High Affinity
The high affinity of the Mouse Anti-APP Recombinant Antibody ensures that we get strong, clear signals even with low protein concentrations. Highly recommended for sensitive assays.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-401, RRID: AB_3111653)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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