What is the specificity of Mouse Anti-APP Recombinant Antibody, clone 10D5?

The Mouse Anti-APP Recombinant Antibody, clone 10D5, is highly specific for amyloid precursor protein (APP).

Is this antibody validated for use on human samples?

Yes, this antibody has been validated for use on human samples.

Mouse Anti-APP Recombinant Antibody (clone 10D5)

CAT#: PABL-401

Recombinant Mouse Antibody (10D5) is capable of binding to APP. This antibody recognizes Aβ residues 3–7.

Published Data Gene Expression

Figure 1 Application of anti-amyloid antibody 10D5 clears amyloid-β plaques.

Amyloid-β plaque clearance occurred only in cortical tissue treated with 10D5. Amyloid protein was immunostained with fluorescent 3D6. Untreated area of cortexin animal treated with 10D5. Plaques are clearly visible (arrow). Scale bar, 50μm.

Lombardo, J. A., Stern, E. A., McLellan, M. E., Kajdasz, S. T., Hickey, G. A., Bacskai, B. J., & Hyman, B. T. (2003). Amyloid-β antibody treatment leads to rapid normalization of plaque-induced neuritic alterations. Journal of Neuroscience, 23(34), 10879-10883.

FuncS

Figure 2 Application of anti-amyloid antibody 10D5 clears amyloid-β plaques.

Comparison of amyloid burden measured with 3D6 antibody under different experimental conditions. The values are the means; error bars indicate SD.

Figure 3 Amyloid-β clearance restores the morphology of neurites.

Untreated area of cortex from 10D5-treated animal, immunostained with Cy-3-SMI-32.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/60_E2_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/61_E2_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/157/2073_B_7_5.jpg

RNA Expression Figure 5 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142192-APP

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Specifications

Immunogen
Human amyloid beta (A4) precursor protein

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG2a

Specificity
APP

Species Reactivity
Human

Clone
10D5

Applications
IF, FuncS

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Immunofluorescence and Function Assay.

Target

Alternative Names
APP; amyloid beta (A4) precursor protein; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma; amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); alzheimer disease amyloid protein; cerebral vascular amyloid peptide

Gene ID
351

UniProt ID
P05067

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Breast cancer biomarkers at key points during disease progression

Extremely Specific

The Mouse Anti-APP Recombinant Antibody (clone 10D5) exhibits outstanding specificity in our Western blot experiments. It detects APP without cross-reacting with other proteins, making it indispensable in our research.

Well Cited

The fact that this antibody is widely cited in peer-reviewed literature gave us confidence in its utility. It has lived up to the reputation, providing robust results for our APP studies.

High Affinity

The high affinity of the Mouse Anti-APP Recombinant Antibody ensures that we get strong, clear signals even with low protein concentrations. Highly recommended for sensitive assays.

Q&As

What is the specificity of Mouse Anti-APP Recombinant Antibody, clone 10D5?

A: The Mouse Anti-APP Recombinant Antibody, clone 10D5, is highly specific for amyloid precursor protein (APP).
Is the isotype of the antibody known?

A: Yes, the isotype is IgG2a.
Is this antibody validated for use on human samples?

A: Yes, this antibody has been validated for use on human samples.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABL-401, RRID: AB_3111653)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABL-401.pdf

MSDS

MSDS - PABL-401.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Clone 10D5"

CAT
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
TAB-0343CL	Human Anti-APP Recombinant Antibody (TAB-0343CL)	ELISA, Inhib	Human IgG
TAB-0783CLV-F(E)	Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0783CLV-F(E))	ELISA, FuncS	Human Fab
TAB-0784CLV-F(E)	Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0784CLV-F(E))	ELISA	Human Fab
TAB-0785CLV-F(E)	Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0785CLV-F(E))	ELISA	Human Fab
TAB-0786CLV-F(E)	Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0786CLV-F(E))	ELISA	Human Fab

CAT	Product Name	Application	Type
TAB-254CQ	Human Anti-APP Recombinant Antibody (TAB-254CQ)	ELISA	Chimeric (mouse/human) IgG1
TAB-0789CLV	Human Anti-APP Recombinant Antibody (TAB-0789CLV)	IP	Chimeric (mouse/human) IgG
TAB-0802CLV	Human Anti-APP Recombinant Antibody (TAB-0802CLV)	ELISA	Chimeric (mouse/human) IgG1, κ
TAB-0789CLV-F(E)	Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0789CLV-F(E))	ELISA	Chimeric (mouse/human) Fab
TAB-0802CLV-F(E)	Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-0802CLV-F(E))	ELISA	Chimeric (mouse/human) Fab

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TAB-252CQ-F(E)	Mouse Anti-APP Recombinant Antibody; Fab Fragment (TAB-252CQ-F(E))	ELISA	Mouse Fab
TAB-255CQ-F(E)	Mouse Anti-APP Recombinant Antibody; Fab Fragment (TAB-255CQ-F(E))	ELISA	Mouse Fab
TAB-0772CLV-S(P)	Mouse Anti-APP Recombinant Antibody; scFv Fragment (TAB-0772CLV-S(P))	ELISA	Mouse scFv
TAB-0773CLV-S(P)	Mouse Anti-APP Recombinant Antibody; scFv Fragment (TAB-0773CLV-S(P))	ELISA	Mouse scFv
TAB-0774CLV-S(P)	Mouse Anti-APP Recombinant Antibody; scFv Fragment (TAB-0774CLV-S(P))	ELISA	Mouse scFv

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MOR-0082-FY	Rabbit Anti-APP Recombinant Antibody (clone AFY0053)	ICC, IHC-P, WB	Rabbit IgG

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EPAF-0566CQ	Mouse Anti-APP Recombinant Antibody (clone 6E10)	ELISA	Mouse IgG
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EPAF-0568CQ	Human Anti-APP Recombinant Antibody (clone HyL5)	ELISA, Inhib	Human IgM
EPAF-0569CQ	Mouse Anti-APP Recombinant Antibody (clone AB1-42)	IHC	Mouse IgG
EPAF-0570CQ	Human Anti-APP Recombinant Antibody (clone L11.3)	IHC, ELISA, Inhib	Human IgM

CAT	Product Name	Application	Type
NS-001CN	Human Anti-APP Recombinant Antibody (clone AS)	WB	Human IgG
NS-007CN	Mouse Anti-APP Recombinant Antibody (NS-007CN)	ELISA, FuncS	Mouse IgG1
NS-001CN-F(E)	Human Anti-APP Recombinant Antibody (clone AS); Fab Fragment	WB	Human Fab
NS-007CN-F(E)	Mouse Anti-APP Recombinant Antibody; Fab Fragment (NS-007CN-F(E))	ELISA	Mouse Fab
NS-001CN-S(P)	Human Anti-APP Recombinant Antibody (clone AS); scFv Fragment	WB	Human scFv

CAT	Product Name	Application	Type
HPAB-0116CQ-S(P)	Mouse Anti-APP Recombinant Antibody; scFv Fragment (HPAB-0116CQ-S(P))	ELISA, WB, IP, FuncS	Mouse scFv
HPAB-0117CQ-S(P)	Human Anti-APP Recombinant Antibody; scFv Fragment (HPAB-0117CQ-S(P))	ELISA, WB, IP, FuncS	Human scFv
HPAB-0118CQ-S(P)	Human Anti-APP Recombinant Antibody (clone Fv1E4); scFv Fragment	ELISA, Inhib	Human scFv
HPAB-0119CQ-S(P)	Human Anti-APP Recombinant Antibody (clone h12A11v1); scFv Fragment	ELISA, FuncS	Human scFv
HPAB-0120CQ-S(P)	Human Anti-APP Recombinant Antibody; scFv Fragment (HPAB-0120CQ-S(P))	ELISA, IHC, FuncS	Human scFv

CAT	Product Name	Application	Type
AFC-TAB-225	Afuco™ Anti-APP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-225)	FuncS, IF, Neut, ELISA, FC	ADCC enhanced antibody
AFC-TAB-228	Afuco™ Anti-APP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-228)	FuncS, IF, Neut, ELISA, FC	ADCC enhanced antibody
AFC-TAB-229	Afuco™ Anti-APP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-229)	FuncS, IF, Neut, ELISA, FC, IP	ADCC enhanced antibody
AFC-TAB-227	Afuco™ Anti-APP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-227)	ELISA, IP, FC, FuncS, Neut	ADCC enhanced antibody
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HPAB-0008-YJ	Mouse Anti-APP Recombinant Antibody (clone 508F)	ELISA	Mouse IgM

CAT	Product Name	Application	Type
HPAB-0008-YJ-F(E)	Mouse Anti-APP Recombinant Antibody (clone 508F); Fab Fragment	ELISA	Mouse Fab
HPAB-0732-FY-S(P)	Mouse Anti-APP Recombinant Antibody; scFv Fragment (HPAB-0732-FY-S(P))	DB, ELISA	Mouse scFv
HPAB-0733-FY-S(P)	Mouse Anti-APP Recombinant Antibody; scFv Fragment (HPAB-0733-FY-S(P))	DB, ELISA	Mouse scFv
HPAB-0735-FY-S(P)	Human Anti-APP Recombinant Antibody (clone H2L1); Fab Fragment	ELISA, FC, WB	Humanized Fab
HPAB-0736-FY-S(P)	Human Anti-APP Recombinant Antibody (clone H51L21); Fab Fragment	ELISA, FC, WB	Humanized Fab

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VS-0424-XY17	AbPlus™ Anti-APP Magnetic Beads (DE2B4)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-1024-XY23	Mouse Anti-NHP APP Recombinant Antibody (VS-1024-XY23)	WB, ICC	Mouse IgG1

CAT	Product Name	Application	Type
VS-0325-FY6	Human Anti-APP (clone 2A7) scFv-Fc Chimera	ELISA	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC204	Recombinant Anti-APP Vesicular Antibody, EV Displayed (VS-0425-YC204)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application
VS-0525-XY469	Anti-APP Immunohistochemistry Kit	IHC
VS-0525-XY470	Anti-Mouse APP Immunohistochemistry Kit	IHC
VS-0525-XY471	Anti-Monkey APP Immunohistochemistry Kit	IHC

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VS-0525-YC2	Recombinant Anti-APP Biparatopic Antibody, Tandem sdAb	ELISA, Inhib	Tandem sdAb
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VS-0525-YC196	Recombinant Anti-APP (AA 1-5 x AA 13-28) Biparatopic Antibody, Tandem scFv	ELISA, FC, IF, IP, Neut, WB	Tandem scFv
VS-0525-YC198	Recombinant Anti-APP (AA 13-28 x AA 33-40) Biparatopic Antibody, Tandem scFv (Solanezumab x Ponezumab)	ELISA, FC, IF, IP, Neut, WB	Tandem scFv

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