Can this antibody be utilized in western blot and IF?

Yes, the humanized anti-Abeta recombinant antibody is proven in both western blot and immunofluorescence applications. Its purity (>97% by SDS-PAGE) and specificity for Abeta provide robust and consistent binding, delivering reliable data in various in vitro experiments focused on protein aggregation.

What ensures its robust performance in research assays?

The antibody's robust performance is ensured by its humanized design, rigorous purification, and validation across several immunoassay platforms such as WB, ELISA, and functional studies. Its high purity and specific binding to Abeta make it an indispensable tool for exploring protein aggregation in research.

Anti-Human Abeta Recombinant Antibody (Solanezumab)

CAT#: TAB-228

Recombinant monoclonal antibody to Abeta. It binds to the amyloid-β peptides that make up the protein plaques seen in the brains of people with the disease.

Tested Data Gene Expression

SDS-PAGE

Figure 1 Anti-Human Abeta Antibody in SDS-PAGE

SDS-PAGE analysis of TAB-228 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/60_E2_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/61_E2_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/157/2073_B_7_5.jpg

RNA Expression Figure 5 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142192-APP

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Mouse

Derivation
Humanized (from mouse)

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
WB, FuncS, IF, Neut, ELISA, FC, IP

CAS
955085-14-0

Generic Name
Solanezumab

UNII
5D6PWO0333

MW
144.1 kDa

Related Disease
Alzheimer's disease (AD)

Product Property

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage
Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.

Applications

Application Notes
The APP antibody has been reported in applications of FuncS.

Target

Alternative Names
Solanezumab;955085-14-0;LY2062430;m266;10D5;APP;amyloid beta (A4) precursor protein;AD1, Alzheimer disease;amyloid beta A4 protein;peptidase nexin II;preA4;protease nexin-II;peptidase nexin-II;beta-amyloid peptide;alzheimer disease amyloid protein;cerebra

Gene ID
351

UniProt ID
P05067

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Breast cancer biomarkers at key points during disease progression

Excellent Abeta Tool

I have used the humanized anti-Abeta recombinant antibody in immunoblot and immunofluorescence studies. The antibody reliably recognizes Abeta with high specificity and purity (>97%), delivering consistent results and improving the clarity of protein aggregation analysis in my research.

Reliable Abeta Probe

The anti-Abeta recombinant antibody offers impressive specificity for Abeta detection in various research applications such as WB and functional studies. The high quality of the humanized antibody and its validated performance under reducing conditions have solidified its value in our protein aggregation investigations.

Q&As

Can this antibody be utilized in western blot and IF?

A: Yes, the humanized anti-Abeta recombinant antibody is proven in both western blot and immunofluorescence applications. Its purity (>97% by SDS-PAGE) and specificity for Abeta provide robust and consistent binding, delivering reliable data in various in vitro experiments focused on protein aggregation.
What ensures its robust performance in research assays?

A: The antibody's robust performance is ensured by its humanized design, rigorous purification, and validation across several immunoassay platforms such as WB, ELISA, and functional studies. Its high purity and specific binding to Abeta make it an indispensable tool for exploring protein aggregation in research.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-228, RRID: AB_3111886)

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Biosimilar Overview

Please refer to Solanezumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Solanezumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-228.pdf

MSDS

MSDS - TAB-228.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Solanezumab"

Afuco™ Anti-APP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-228)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Abeta. It binds to the amyloid-β peptides that make up the protein plaques seen in the brains of people with the disease.

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TAB-0019CL-VHH	Camelid Anti-APP Recombinant Antibody (TAB-0019CL-VHH)	IHC, WB	Camelid VHH

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TAB-253CQ	Human Anti-APP Recombinant Antibody (TAB-253CQ)	ELISA	Humanized IgG1

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TAB-254CQ	Human Anti-APP Recombinant Antibody (TAB-254CQ)	ELISA	Chimeric (mouse/human) IgG1

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TAB-253CQ-S(P)	Human Anti-APP Recombinant Antibody; scFv Fragment (TAB-253CQ-S(P))	ELISA	Humanized scFv

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TAB-254CQ-S(P)	Mouse Anti-APP Recombinant Antibody; scFv Fragment (TAB-254CQ-S(P))	ELISA	Mouse scFv

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TAB-254CQ-F(E)	Human Anti-APP Recombinant Antibody; Fab Fragment (TAB-254CQ-F(E))	ELISA	Chimeric (mouse/human) Fab

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TAB-0789CLV	Human Anti-APP Recombinant Antibody (TAB-0789CLV)	IP	Chimeric (mouse/human) IgG

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TAB-0802CLV	Human Anti-APP Recombinant Antibody (TAB-0802CLV)	ELISA	Chimeric (mouse/human) IgG1, κ

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MOR-0220	Hi-Affi™ Rabbit Anti-APP Recombinant Antibody (clone DS220AB)	ELISA, WB	Rabbit IgG

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EPAF-0568CQ	Human Anti-APP Recombinant Antibody (clone HyL5)	ELISA, Inhib	Human IgM

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NS-007CN	Mouse Anti-APP Recombinant Antibody (NS-007CN)	ELISA, FuncS	Mouse IgG1

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NS-007CN-F(E)	Mouse Anti-APP Recombinant Antibody; Fab Fragment (NS-007CN-F(E))	ELISA	Mouse Fab

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AFC-TAB-225	Afuco™ Anti-APP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-225)	FuncS, IF, Neut, ELISA, FC	ADCC enhanced antibody

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AFC-TAB-229	Afuco™ Anti-APP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-229)	FuncS, IF, Neut, ELISA, FC, IP	ADCC enhanced antibody

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AFC-TAB-227	Afuco™ Anti-APP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-227)	ELISA, IP, FC, FuncS, Neut	ADCC enhanced antibody

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HPAB-0008-YJ	Mouse Anti-APP Recombinant Antibody (clone 508F)	ELISA	Mouse IgM

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MOR-0082-FY	Rabbit Anti-APP Recombinant Antibody (clone AFY0053)	ICC, IHC-P, WB	Rabbit IgG

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VS-0424-XY17	AbPlus™ Anti-APP Magnetic Beads (DE2B4)	IP, Protein Purification

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VS-1024-XY23	Mouse Anti-NHP APP Recombinant Antibody (VS-1024-XY23)	WB, ICC	Mouse IgG1

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VS-0325-FY6	Human Anti-APP (clone 2A7) scFv-Fc Chimera	ELISA	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC204	Recombinant Anti-APP Vesicular Antibody, EV Displayed (VS-0425-YC204)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY469	Anti-APP Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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VS-0525-YC18	Recombinant Anti-APP (AA 3-7 x AA 12-28) Biparatopic Antibody, Tandem scFv (Clone h12A11v1 x Solanezumab)	ELISA, Neut, FC, IF, IP, WB	Tandem scFv

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VS-0525-YC196	Recombinant Anti-APP (AA 1-5 x AA 13-28) Biparatopic Antibody, Tandem scFv	ELISA, FC, IF, IP, Neut, WB	Tandem scFv

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VS-0525-YC198	Recombinant Anti-APP (AA 13-28 x AA 33-40) Biparatopic Antibody, Tandem scFv (Solanezumab x Ponezumab)	ELISA, FC, IF, IP, Neut, WB	Tandem scFv

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VS-0525-XY471	Anti-Monkey APP Immunohistochemistry Kit	IHC

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