Is this anti-APP recombinant antibody clone 3D6 suitable for Western blotting?

Yes, the anti-APP recombinant antibody clone 3D6 (PABL-011) is suitable for Western blotting applications. It provides specific binding to APP, making it a reliable choice for such assays.

What are the recommended storage conditions for the anti-APP recombinant antibody clone 3D6?

The recommended storage condition for the anti-APP recombinant antibody clone 3D6 (PABL-011) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To maintain stability, avoid repeated freeze-thaw cycles.

Can the anti-APP recombinant antibody clone 3D6 be used in immunoprecipitation assays?

Yes, the anti-APP recombinant antibody clone 3D6 (PABL-011) can be used in immunoprecipitation assays. It is designed to effectively bind to APP, enabling the successful precipitation of the target protein from complex mixtures.

Is this anti-APP recombinant antibody clone 3D6 effective in ELISA applications?

Yes, the anti-APP recombinant antibody clone 3D6 (PABL-011) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of APP.

What is the optimal dilution for using the anti-APP recombinant antibody clone 3D6 in immunofluorescence?

The optimal dilution for using the anti-APP recombinant antibody clone 3D6 (PABL-011) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for specific experimental conditions.

Mouse Anti-APP Recombinant Antibody (clone 3D6)

CAT#: PABL-011

Recombinant Mouse Antibody (3D6) is capable of binding to APP. It is a neo-epitope specific antibody recognizing amyloid-β1-5 with strong preference for an exposed Asp residue at the N-terminus of the peptide.

Published Data Gene Expression

Figure 1 Neural Activity Controls Formationof APP Cleavage Derivatives.

Western blots of extracts from organotypic APPSWE slices in the presence of -secretase inhibitor 1 M L-685,458.

Kamenetz, F., Tomita, T., Hsieh, H., Seabrook, G., Borchelt, D., Iwatsubo, T., ... & Malinow, R. (2003). APP processing and synaptic function. Neuron, 37(6), 925-937.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/60_E2_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/61_E2_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/157/2073_B_7_5.jpg

RNA Expression Figure 5 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142192-APP

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Specifications

Immunogen
Human amyloid beta precursor protein

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG1

Specificity
Human APP

Species Reactivity
Human

Clone
3D6

Applications
WB, ELISA, FuncS

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody 3D6 has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.

Target

Alternative Names
AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma

Gene ID
351

UniProt ID
P05067

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Customer Review

There are currently no Customer reviews or questions for PABL-011. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Emily Johnson

Excellent for IHC

I've been using the Anti-APP Recombinant Antibody (clone 3D6) for my immunohistochemistry experiments, and it has delivered excellent results. The staining of amyloid plaques in mouse brain tissue is clear and specific, which has greatly improved the accuracy of my assessments. Consistent performance across different batches has also been a huge plus, reducing troubleshooting time significantly. The high quality of this antibody has made my work much more efficient and reliable.

John Smith

Highly Dependable for WB

We recently incorporated this antibody into our Western blot and immunoprecipitation assays, and it's been a great addition to our lab. The specificity and sensitivity are outstanding, consistently detecting APP and its fragments with high clarity. This reliability has enabled us to focus more on our research rather than worrying about variability or non-specific binding.

Sarah Williams

Reliable in Flow Cytometry

The antibody has proven to be incredibly reliable in our flow cytometry and immunofluorescence applications. The results are always high-quality and reproducible, with strong affinity and specificity for APP. It has become a staple in our lab for various experiments, providing us with the confidence to proceed with complex assays.

Sama***a

ELISA for PABL-011

This antibody works well in ELISA with β-Amyloid (1-42).

Q&As

Is this anti-APP recombinant antibody clone 3D6 suitable for Western blotting?

A: Yes, the anti-APP recombinant antibody clone 3D6 (PABL-011) is suitable for Western blotting applications. It provides specific binding to APP, making it a reliable choice for such assays.
What are the recommended storage conditions for the anti-APP recombinant antibody clone 3D6?

A: The recommended storage condition for the anti-APP recombinant antibody clone 3D6 (PABL-011) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To maintain stability, avoid repeated freeze-thaw cycles.
Can the anti-APP recombinant antibody clone 3D6 be used in immunoprecipitation assays?

A: Yes, the anti-APP recombinant antibody clone 3D6 (PABL-011) can be used in immunoprecipitation assays. It is designed to effectively bind to APP, enabling the successful precipitation of the target protein from complex mixtures.
Is this anti-APP recombinant antibody clone 3D6 effective in ELISA applications?

A: Yes, the anti-APP recombinant antibody clone 3D6 (PABL-011) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of APP.
What is the optimal dilution for using the anti-APP recombinant antibody clone 3D6 in immunofluorescence?

A: The optimal dilution for using the anti-APP recombinant antibody clone 3D6 (PABL-011) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

Maniv, Inbal, et al. "Altered ubiquitin signaling induces Alzheimer's disease-like hallmarks in a three-dimensional human neural cell culture model." Nature Communications 14.1 (2023): 5922. https://doi.org/10.1038/s41467-023-41545-7

The study investigates the role of altered ubiquitin signaling in inducing Alzheimer's disease (AD)-like hallmarks using a three-dimensional human neural cell culture model. It highlights that the accumulation of the UBB+1 protein is an early event in Alzheimer's disease. The research demonstrates that UBB+1 expression induces AD pathology in human neurons by interacting with UCHL1 and altering APP protein levels. Notably, silencing UBB+1 expression rescues AD pathology in human familial AD (FAD) neurons.
Creative Biolabs significantly contributed to this study by providing the 3D6 anti-amyloid-β antibody (Cat. no. PABL-011), which was used in immunofluorescence staining experiments. The use of this antibody was crucial for detecting amyloid-β aggregates, a key pathological feature of Alzheimer's disease, thus enabling the researchers to better understand and visualize the AD-like pathology in their 3D neural cell culture model.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABL-011, RRID: AB_3111619)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABL-011.pdf

MSDS

MSDS - PABL-011.pdf

COA

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