Mouse Anti-APP Recombinant Antibody (clone 3D6) (CAT#: PABL-011)
Recombinant Mouse Antibody (3D6) is capable of binding to APP. It is a neo-epitope specific antibody recognizing amyloid-β1-5 with strong preference for an exposed Asp residue at the N-terminus of the peptide.
Specific Inquiry
WB
Figure 1 Neural Activity Controls Formationof APP Cleavage Derivatives.
Western blots of extracts from organotypic APPSWE slices in the presence of -secretase inhibitor 1 M L-685,458.
Kamenetz, F., Tomita, T., Hsieh, H., Seabrook, G., Borchelt, D., Iwatsubo, T., ... & Malinow, R. (2003). APP processing and synaptic function. Neuron, 37(6), 925-937.
Specifications
- Immunogen
- Human amyloid beta precursor protein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human APP
- Species Reactivity
- Human
- Clone
- 3D6
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 3D6 has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
- Gene ID
- 351
- UniProt ID
- P05067
Related Resources
Maniv, Inbal, et al. "Altered ubiquitin signaling induces Alzheimer's disease-like hallmarks in a three-dimensional human neural cell culture model." Nature Communications 14.1 (2023): 5922. https://doi.org/10.1038/s41467-023-41545-7
The study investigates the role of altered ubiquitin signaling in inducing Alzheimer's disease (AD)-like hallmarks using a three-dimensional human neural cell culture model. It highlights that the accumulation of the UBB+1 protein is an early event in Alzheimer's disease. The research demonstrates that UBB+1 expression induces AD pathology in human neurons by interacting with UCHL1 and altering APP protein levels. Notably, silencing UBB+1 expression rescues AD pathology in human familial AD (FAD) neurons.
Creative Biolabs significantly contributed to this study by providing the 3D6 anti-amyloid-β antibody (Cat. no. PABL-011), which was used in immunofluorescence staining experiments. The use of this antibody was crucial for detecting amyloid-β aggregates, a key pathological feature of Alzheimer's disease, thus enabling the researchers to better understand and visualize the AD-like pathology in their 3D neural cell culture model.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-016 | Recombinant Anti-Human APP VHH Single Domain Antibody | CA, IP, IF, FuncS | Llama VHH |
| PNBL-003 | Recombinant Anti-Human APP VHH Single Domain Antibody (PNBL-003) | WB, ELISA, FuncS | Llama VHH |
| PNBL-004 | Recombinant Anti-Human APP VHH Single Domain Antibody (PNBL-004) | WB, ELISA, FuncS | Llama VHH |
| TAB-0019CL-VHH | Camelid Anti-APP Recombinant Antibody (TAB-0019CL-VHH) | IHC, WB | Camelid VHH |
| TAB-0020CL-VHH | Anti-Human APP VHH Single Domain Antibody (TAB-0020CL-VHH) | ELISA, IHC | Single domain antibody |
Customer Reviews and Q&As
Customer Review
Q&As
Emily Johnson
Excellent for IHC
Excellent for IHC
I've been using the Anti-APP Recombinant Antibody (clone 3D6) for my immunohistochemistry experiments, and it has delivered excellent results. The staining of amyloid plaques in mouse brain tissue is clear and specific, which has greatly improved the accuracy of my assessments. Consistent performance across different batches has also been a huge plus, reducing troubleshooting time significantly. The high quality of this antibody has made my work much more efficient and reliable.
15-Aug-23
John Smith
Highly Dependable for WB
Highly Dependable for WB
We recently incorporated this antibody into our Western blot and immunoprecipitation assays, and it's been a great addition to our lab. The specificity and sensitivity are outstanding, consistently detecting APP and its fragments with high clarity. This reliability has enabled us to focus more on our research rather than worrying about variability or non-specific binding.
23-Nov-22
Sarah Williams
Reliable in Flow Cytometry
Reliable in Flow Cytometry
The antibody has proven to be incredibly reliable in our flow cytometry and immunofluorescence applications. The results are always high-quality and reproducible, with strong affinity and specificity for APP. It has become a staple in our lab for various experiments, providing us with the confidence to proceed with complex assays.
30-Jul-21
Sama***a
ELISA for PABL-011
ELISA for PABL-011
This antibody works well in ELISA with β-Amyloid (1-42).
29-Feb-24
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-011, RRID: AB_3111619)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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