Recombinant Human Anti-GCGR Antibody (mAb1) (CAT#: PABL-480)
Recombinant Human Antibody (mAb1) is capable of binding to GCGR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-GCGR mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-GCGR proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The mechanisms of glucagon receptor inhibition by blocking antibodies targeting the receptor's extracellular domain (ECD).
Specific Inquiry
Figure 1 Western blot analysis of stable 293 cells expressing WT or G40S GCGR, probed with anti-GCGR mAb1.
WT or G40S -ve refers to cells incubated without anti-GCGR.
Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.
Figure 2 FACS analysis of stable 293 cells expressing WT or G40S GCGR using anti-GCGR mAb1.
WT or G40S -ve refers to cells incubated without anti-GCGR.
Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.
Specifications
- Immunogen
- Human glucagon receptor
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native GCGR
- Species Reactivity
- Human
- Clone
- mAb1
- Applications
- WB 1:1000-1:10000
ELISA 1:1000-1:20000
FC Assay-depended
FuncS Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-099 | Mouse Anti-GCGR Recombinant Antibody (clone mAb7); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| PFBW-048 | Mouse Anti-GCGR Recombinant Antibody (clone HGR-2 F6); Fab Fragment | Block | Mouse Fab |
| HPAB-1620LY-F(E) | Human Anti-GCGR Recombinant Antibody (clone CBL034); Fab Fragment | ELISA, IHC | Human Fab |
| HPAB-1621LY-F(E) | Human Anti-GCGR Recombinant Antibody (clone CBL035); Fab Fragment | ELISA, IHC | Human Fab |
| HPAB-1622LY-F(E) | Human Anti-GCGR Recombinant Antibody (clone CBL036); Fab Fragment | ELISA, IHC | Human Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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