Recombinant Human Anti-GCGR Antibody (mAb1)
CAT#: PABL-480
Recombinant Human Antibody (mAb1) is capable of binding to GCGR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-GCGR mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-GCGR proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The mechanisms of glucagon receptor inhibition by blocking antibodies targeting the receptor's extracellular domain (ECD).
Published Data
Gene Expression
Figure 1 Western blot analysis of stable 293 cells expressing WT or G40S GCGR, probed with anti-GCGR mAb1.
WT or G40S -ve refers to cells incubated without anti-GCGR.
Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.
Figure 2 FACS analysis of stable 293 cells expressing WT or G40S GCGR using anti-GCGR mAb1.
WT or G40S -ve refers to cells incubated without anti-GCGR.
Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71228/1338_A11_7_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HeLa, MCF7, SK-BR-3, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000215644-GCGR
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Specifications
- Immunogen
- Human glucagon receptor
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native GCGR
- Species Reactivity
- Human
- Clone
- mAb1
- Applications
- WB 1:1000-1:10000
ELISA 1:1000-1:20000
FC Assay-depended
FuncS Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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|---|---|---|---|
| PFBW-048 | Mouse Anti-GCGR Recombinant Antibody (clone HGR-2 F6); Fab Fragment | Block | Mouse Fab |
| PFBL-477 | Recombinant Human Anti-GCGR Antibody Fab Fragment (PFBL-477) | WB, ELISA, FuncS | Fab |
| HPAB-0023-LS-S(P) | Mouse Anti-GCGR Recombinant Antibody (clone 6.23V3); scFv Fragment | WB, FC | Mouse scFv |
| HPAB-0138-FY-F(E) | Human Anti-GCGR Recombinant Antibody; Fab Fragment (HPAB-0138-FY-F(E)) | Block | Human Fab |
| HPAB-3045LY-F(E) | Human Anti-GCGR Recombinant Antibody; Fab Fragment (HPAB-3045LY-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-048 | Mouse Anti-GCGR Recombinant Antibody (clone HGR-2 F6); scFv Fragment | Block | Mouse scFv |
| PSBL-477 | Recombinant Human Anti-GCGR Antibody scFv Fragment (PSBL-477) | WB, ELISA, FuncS | scFv |
| HPAB-3385LY-S(P) | Human Anti-GCGR Recombinant Antibody (clone CBL150); scFv Fragment | ELISA | Human scFv |
| HPAB-3386LY-S(P) | Human Anti-GCGR Recombinant Antibody (clone CBL151); scFv Fragment | ELISA | Human scFv |
| HPAB-3387LY-S(P) | Human Anti-GCGR Recombinant Antibody (clone CBL152); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-065CQ | Anti-Human GCGR Recombinant Antibody (H6.23v3) | ELISA, FC, WB | Human antibody |
| TAB-068CQ | Human Anti-GCGR Recombinant Antibody (TAB-068CQ) | ELISA, FC | Human IgG2 |
| TAB-065CQ-F(E) | Anti-Human GCGR Recombinant Antibody Fab Fragment (H6.23v3) | ELISA, FC, WB | Human antibody |
| TAB-068CQ-F(E) | Human Anti-GCGR Recombinant Antibody; Fab Fragment (TAB-068CQ-F(E)) | ELISA, FC | Human Fab |
| TAB-069CQ-F(E) | Human Anti-GCGR Recombinant Antibody; Fab Fragment (TAB-069CQ-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-066CQ | Anti-Human GCGR Recombinant Antibody (3C7v11) | ELISA, FC, WB | Humanized antibody |
| TAB-066CQ-S(P) | Anti-Human GCGR Recombinant Antibody scFv Fragment (3C7v11) | ELISA, FC, WB | Humanized antibody |
| TAB-066CQ-F(E) | Anti-Human GCGR Recombinant Antibody Fab Fragment (3C7v11) | ELISA, FC, WB | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-063CQ-S(P) | Anti-Human GCGR Recombinant Antibody scFv Fragment (mAb5) | ELISA, FC | |
| TAB-064CQ-S(P) | Anti-Human GCGR Recombinant Antibody scFv Fragment (3C7) | ELISA, FC, WB | |
| TAB-067CQ-S(P) | Mouse Anti-GCGR Recombinant Antibody; scFv Fragment (TAB-067CQ-S(P)) | ELISA, FC | Mouse scFv |
| TAB-063CQ-F(E) | Anti-Human GCGR Recombinant Antibody Fab Fragment (mAb5) | ELISA, FC | |
| PABX-067-F (E) | Recombinant Mouse Anti-GR-2 Antibody Fab Fragment (F6) | WB, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-113LC | Recombinant Anti-Human GCGR Antibody (Fc glycosylation) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY112 | Human Anti-GCGR scFv-Fc Chimera (VS-0425-FY112) | Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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