Recombinant Human Anti-GCGR Antibody (mAb1) (CAT#: PABL-480)

Recombinant Human Antibody (mAb1) is capable of binding to GCGR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-GCGR mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-GCGR proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The mechanisms of glucagon receptor inhibition by blocking antibodies targeting the receptor's extracellular domain (ECD).


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Figure 1 Western blot analysis of stable 293 cells expressing WT or G40S GCGR, probed with anti-GCGR mAb1.

Figure 1 Western blot analysis of stable 293 cells expressing WT or G40S GCGR, probed with anti-GCGR mAb1.

WT or G40S -ve refers to cells incubated without anti-GCGR.

Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.

Figure 2 FACS analysis of stable 293 cells expressing WT or G40S GCGR using anti-GCGR mAb1.

Figure 2 FACS analysis of stable 293 cells expressing WT or G40S GCGR using anti-GCGR mAb1.

WT or G40S -ve refers to cells incubated without anti-GCGR.

Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.


Specifications

  • Immunogen
  • Human glucagon receptor
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native GCGR
  • Species Reactivity
  • Human
  • Clone
  • mAb1
  • Applications
  • WB 1:1000-1:10000
    ELISA 1:1000-1:20000
    FC Assay-depended
    FuncS Assay-depended

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • GCGR; glucagon receptor; GGR; GL-R

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone mAb1"

See other products for "GCGR"

Humanized Antibody

CAT Product Name Application Type
TAB-066CQ Anti-Human GCGR Recombinant Antibody (3C7v11) ELISA, FC, WB Humanized antibody
TAB-066CQ-S(P) Anti-Human GCGR Recombinant Antibody scFv Fragment (3C7v11) ELISA, FC, WB Humanized antibody
TAB-066CQ-F(E) Anti-Human GCGR Recombinant Antibody Fab Fragment (3C7v11) ELISA, FC, WB Humanized antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-113LC Recombinant Anti-Human GCGR Antibody (Fc glycosylation) ELISA Chimeric antibody (mouse/human)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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