Anti-Human GCGR Recombinant Antibody Fab Fragment (mAb5) (CAT#: TAB-063CQ-F(E))
The antibody recognizes a functional epitope on human glucagon receptor and can be used to lower glucose levels in blood. It can be potentially used in the prevention and treatment of glucose-related disorders, including diabetes, hyperglycemia, hyperinsulinemia and so on.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71228/1338_A11_7_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71228/1338_A11_7_selected.jpg
Figure 2 RNA cell line category: Cell line enhanced (HeLa, MCF7, SK-BR-3, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000215644-GCGR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000215644-GCGR
Specifications
- Immunogen
- Human glucagon receptor
- Host Species
- Mouse
- Specificity
- Human
- Species Reactivity
- Human, Cynomolgus monkey
- Clone
- mAb5
- Applications
- ELISA, FC, FuncS
- Related Disease
- Type 2 Diabetes
Applications
- Application Notes
- Cell Based Functional Assay
cAMP signaling was measured in a CHO cell line stably transfected with human glucagon receptor, using a LANCE® cAMP kit. Cells were added to a 96-well plate and incubated with serially diluted mAb5. Cells were then stimulated with 200 pM glucagon for 30 minutes at room temperature (RT). Detection antibody was then added and incubated for 10 minutes at RT. Cells were then lysed with supplied detection buffer and incubated in the dark for 3 hours at RT. The plate was then read on a VICTOR3™ plate reader. As the readout of this assay is based on competition between a labeled, exogenous cAMP (measured at 665 nm) and unlabeled, endogenously generated cAMP, higher signals indicate less cAMP generated by the cells. mAb5 inhibits cAMP generation in a dose-dependent fashion.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-480 | Recombinant Human Anti-GCGR Antibody (mAb1) | WB, ELISA, FuncS | IgG |
| HPAB-1620LY | Human Anti-GCGR Recombinant Antibody (clone CBL034) | ELISA, IHC | Human IgG |
| HPAB-1621LY | Human Anti-GCGR Recombinant Antibody (clone CBL035) | ELISA, IHC | Human IgG |
| HPAB-1622LY | Human Anti-GCGR Recombinant Antibody (clone CBL036) | ELISA, IHC | Human IgG |
| ZG-0142U | Human Anti-GCGR Recombinant Antibody (clone 9G1) | ELISA | Humanized IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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