Anti-Human IL17A Recombinant Antibody (TAB-069) (CAT#: TAB-069)
Recombinant monoclonal antibody to IL17A. It is a human monoclonal antibody designed for the treatments of uveitis, rheumatoid arthritis, and psoriasis. It targets member A from the cytokine family of interleukin 17.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-Human IL17A Antibody (TAB-069) in ELISA
ELISA analysis of TAB-069 was performed by coating with Human IL17A Protein (No Tag). Then blocked with BSA and incubated with TAB-069. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-Human IL17A Antibody (TAB-069) in WB
Western blot analysis of TAB-069 was performed with Human IL17A Protein (No Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-069 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 3 Anti-Human IL17A Antibody (TAB-069) in DB
Dot Blot analysis of TAB-069 was performed by coating with Human IL17A Protein (No Tag).
TAB-069 incubation concentration: 2 μg/mL.
HRP goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
Specifications
- Immunogen
- HuIL-17 coupled 10 KLH mixed 1:1 with huIL-17 in Gerbu adjuvant.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG1 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- IF, IHC, ELISA, FC
- MW
- 147.94 kDa
- Related Disease
- Psoriatic arthritis (PSA)
Product Property
- Purity
- >95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- At -20°C for one year.
Applications
- Application Notes
- The IL17A antibody has been reported in applications of Immunofluorescence, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Flow Cytometry. FC: The isotype, IL17RA AlexaFluor 647 and IL17RC AlexaFluor 488 antibodies were used at 4mg/ml and 10mg/ml respectively for FACs analysis.
Target
- Alternative Names
- Secukinumab;Cosentyx;875356-43-7;AIN457;DB09029IL17A;interleukin 17A;CTLA8, IL17, interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8);interleukin-17A;cytotoxic T lymphocyte associated protein 8;IL 17;IL 17A;CTLA-8;cytotoxic T-lymphocyte-a
- Gene ID
- 3605
- UniProt ID
- Q16552
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "IL17A"
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H57 | Anti-Human IL17A Recombinant Antibody (Perakizumab) | WB, ELISA, IP, FC, FuncS, Neut, IF | IgG1 - kappa |
TAB-H08 | Anti-Human IL17A/IL17F Recombinant Antibody (TAB-H08) | ELISA, FC, IP, FuncS, IF, Neut, WB | IgG1 - kappa |
TAB-048ZJ | Human Anti-IL17A Recombinant Antibody (TAB-048ZJ) | ELISA, Neut | Humanized antibody |
TAB-048ZJ-S(P) | Human Anti-IL17A Recombinant Antibody; scFv Fragment (TAB-048ZJ-S(P)) | ELISA, Neut | Human scFv |
TAB-458CQ | Anti-Human IL17A Recombinant Antibody (TAB-458CQ) | ELISA, IHC, FC, IP, IF, Inhib | IgG1, κ |
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