Anti-Human IL17A Recombinant Antibody (TAB-069) (CAT#: TAB-069)

Figure 1 Anti-Human IL17A Antibody (TAB-069) in ELISA

ELISA analysis of TAB-069 was performed by coating with Human IL17A Protein (No Tag). Then blocked with BSA and incubated with TAB-069. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human IL17A Antibody (TAB-069) in WB

Western blot analysis of TAB-069 was performed with Human IL17A Protein (No Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-069 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

Figure 3 Anti-Human IL17A Antibody (TAB-069) in DB

Dot Blot analysis of TAB-069 was performed by coating with Human IL17A Protein (No Tag).

TAB-069 incubation concentration: 2 μg/mL.
HRP goat anti-human IgG as a secondary antibody (1: 6000)

NC: Negative control PC: Positive control