Human Anti-IL6 Recombinant Antibody (TAB-0173CL) (CAT#: TAB-0173CL)
This product is a recombinant Human antibody that recognizes IL6. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in ELISA
ELISA analysis of TAB-0173CL was performed by coating with human IL6 protein (His tag). Then blocked with BSA and incubated with TAB-0173CL. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in WB
Western blot analysis of TAB-0173CL was performed by loading human IL6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0173CL and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in DB
Dot Blot analysis of TAB-0173CL was performed by coating with human IL6 protein (His tag).
TAB-0173CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG1
- Specificity
- Human IL6
- Species Reactivity
- Human, Cynomolgus, African green monkey
- Applications
- ELISA, Block, In vivo
- Related Disease
- Diabetic macular edema, wet AMD
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- IL6; interleukin 6; HGF; HSF; BSF2; IL-6; IFNB2; interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
- Gene ID
- 3569
- UniProt ID
- B4DVM1
Related Resources
Inflammatory Bowel Diseases
Rheumatoid Arthritis
Graft-versus-Host Disease
AGE-RAGE Signaling Pathway in Diabetic Complications
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-613 | Recombinant Human Anti-IL-6 Antibody Fab Fragment | ELISA, Neut | Fab |
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| HPAB-588-FY-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-588-FY-F(E)) | ELISA | Human Fab |
| HPAB-591-FY-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-591-FY-F(E)) | ELISA | Human Fab |
| HPAB-0089-WJ-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-0089-WJ-F(E)) | ELISA, WB | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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