Anti-Human IL-6 Recombinant Antibody (Olokizumab) (CAT#: TAB-253)
Recombinant monoclonal antibody to IL-6. Olokizumab is an immunomodulator. It binds to interleukin 6.
Specific Inquiry
ELISA
Figure 1 Anti-Human IL6 Recombinant Antibody (TAB-253) in ELISA
ELISA analysis of TAB-253 was performed by coating with human IL6 protein (His tag). Then blocked with BSA and incubated with TAB-253. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human IL6 Recombinant Antibody (TAB-253) in WB
Western blot analysis of TAB-253 was performed by loading human IL6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-253 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-Human IL6 Recombinant Antibody (TAB-253) in DB
Dot Blot analysis of TAB-253 was performed by coating with human IL6 protein (His tag).
TAB-253 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
SDS-PAGE
Figure 4 Anti-Human IL-6 Recombinant Antibody (TAB-253) in SDS-PAGE
SDS-PAGE analysis of TAB-253 in non-reduced (Lane 1) and reduced (Lane 2) conditions.
SEC-HPLC
Figure 5 Anti-Human IL-6 Recombinant Antibody (TAB-253) in HPLC
HPLC analysis of TAB-253 shows the purity is>95%.
Specifications
- Immunogen
- Recombinant full length protein (Human).
- Host Species
- Rat
- Derivation
- Humanized (from rat)
- Type
- IgG4 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- WB, ELISA, FC, IP, FuncS, IF, Neut
- CAS
- 1007223-17-7
- Generic Name
- Olokizumab
- Related Disease
- Autoimmune diseases
Product Property
- Purity
- >95.0% as determined by analysis by SDS-PAGE.
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The IL6 antibody has been reported in applications of Neut.
MTT: 2,000 cells/well were seeded in 96-well plates in culture medium (RPMI 1640 supplemented with 10% FBS and penicillin/streptomycin) containing different concentrations of siltuximab. After 96 hours of culture, 10 μL MTT (5 mg/mL in PBS) were added to each well and the plates were incubated for 4 hours. The resulting formazan product was dissolved with acid-isopropanol and the absorbance at a wavelength of 490 nm (A490) was read on a SPECTRAmax Microplate Spectrophotometer.
Target
- Alternative Names
- Olokizumab;1007223-17-7;CDP6038;IL6;interleukin 6 (interferon, beta 2);IFNB2;interleukin-6;BSF2;HGF;HSF;IL 6;CDF;BSF-2;IFN-beta-2;interferon beta-2;interleukin BSF-2;hybridoma growth factor;CTL differentiation factor;B-cell stimulatory factor 2;B-cell dif
- Gene ID
- 3569
- UniProt ID
- P05231
Related Resources
Please refer to Olokizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Olokizumab.
Inflammatory Bowel Diseases
Rheumatoid Arthritis
Graft-versus-Host Disease
AGE-RAGE Signaling Pathway in Diabetic Complications
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Olokizumab"
Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-253)This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to IL-6. It is an immunomodulator. It binds to interleukin 6.
DrugMonitor™ Anti-Olokizumab Antibody (VS-1224-YC839)Olokizumab, a humanized anti-IL-6 antibody, is currently under investigation for its potential use in treating autoimmune disorders such as rheumatoid arthritis. The DrugMonitor™ Anti-Olokizumab Antibody (VS-1224-YC839) is an anti-drug antibody (ADA) against Olokizumab. This drug-based antibody is raised in mice immunized with the Olokizumab. The anti-Olokizumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Olokizumab in samples.
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0023CL | Mouse Anti-IL6 Recombinant Antibody (TAB-0023CL) | Elisa, In vivo, FuncS, Block | Mouse IgG |
| TAB-0024CL | Mouse Anti-IL6 Recombinant Antibody (TAB-0024CL) | Elisa, In vivo, FuncS, Block | Mouse IgG |
| TAB-0025CL | Mouse Anti-IL6 Recombinant Antibody (TAB-0025CL) | Elisa, In vivo, FuncS, Block | Mouse IgG |
| TAB-0022CL-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0022CL-S(P)) | Elisa, In vivo, FuncS, Block | Mouse scFv |
| TAB-0023CL-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0023CL-S(P)) | Elisa, In vivo, FuncS, Block | Mouse scFv |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-253, RRID: AB_3111899)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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