Rabbit Anti-MAPT Recombinant Antibody (clone RB86) (CAT#: PABL-713)

Figure 1 Monoclonal antibody DC8E8 inhibits pathological tau–tau interaction.

(A) Tau western blots using MAb86 (top) and human tau-specific antibody HT7 (bottom) for detection. MAb86 binds specifically to ERK-phosphorylated tau (lane 1) but not to unphosphorylated tau (lane 2), ERK-phosphorylated mutant tau S422A (lane 3) or unphosphorylated tau S422A (lane 4). (B) Western blots of brain extracts from TauPS2APP mouse brains using MAb86 (top) and human tau-specific antibody HT7 (bottom) for detection. Extracts were prepared from 16-month-old wild-type mice (lane 1) or triple transgenic mice at 8, 12, 16 and 20 months of age (lanes 2-5). M = molecular weight markers.

Collin, L., Bohrmann, B., Göpfert, U., Oroszlan-Szovik, K., Ozmen, L., & Grüninger, F. (2014). Neuronal uptake of tau/pS422 antibody and reduced progression of tau pathology in a mouse model of Alzheimer‘s disease. Brain, 137(10), 2834-2846.

Figure 2 Tau/pS422 locates in lipid raft microdomains on neuronal membranes in untreated TauPS2APP mice.

(A-D) Pathological tau/ pS422 is detected in lipid raft-like microdomains on the periphery of a dendritic shaft (A and B). (C and D) High magnification of the boxed area in B. Microtubule cytoskeleton is in green (B and D), nuclei are counterstained with DAPI in B. (E-G) Tau/pS422 partially localizes with flotillin 1 as indicated by arrows.

Collin, L., Bohrmann, B., Göpfert, U., Oroszlan-Szovik, K., Ozmen, L., & Grüninger, F. (2014). Neuronal uptake of tau/pS422 antibody and reduced progression of tau pathology in a mouse model of Alzheimer‘s disease. Brain, 137(10), 2834-2846.