Anti-MUC-1 Antibody-Cytokine Fusion Protein, scFv-Fc (C595)-IL2 (CAT#: ACFP-SH017)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-MUC-1 scFv-Fc to IL2. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the MUC-1 as well as the biological activity of IL2. The anti-MUC1-scFv-Fc-IL2 fusion protein that after binding to MUC1-positive tumour cells induces proliferation of peripheral blood T cells and mediates activation of resting NK cells to lysis of MUC1-positive tumour cells. This fusion protein was suitable immunocytokine for the specific immune modulation in the vicinity of MUC1-positive tumours.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RPTEC TERT1
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg
Figure 5 Stomach
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg
Figure 6 Colon
(Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg
(Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg
Figure 7 Kidney
(Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg
(Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg
Figure 8 Testis
(Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg
(Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg
Figure 9 RNA cell line category: Group enriched (CAPAN-2, OE19, RPTEC TERT1, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1
Specifications
- Target
- MUC-1
- Molecule Class
- scFv-Fc-IL2
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- C595
- Related Disease
- MUC1-positive tumours
- Cytokine 1
- IL2
- Cytokines 1 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- MUC1; mucin 1; cell surface associated; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1; transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen
- Gene ID
- 4582
- UniProt ID
- P15941
Downloads
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See other products for "Clone C595"
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2049-VHH | Recombinant Anti-human MUC1 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| HPAB-AP504-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (5-24) | FC | Camel VHH |
| HPAB-AP505-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (aMUC1) | ELISA | Camel VHH |
| HPAB-0736-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (ER46) | ELISA | Camelid VHH |
| HPAB-0737-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (AR-32) | ELISA | Camelid VHH |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H56 | Anti-Human MUC1 Recombinant Antibody (Pemtumomab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG |
| TAB-765 | Mouse Anti-MUC1 Recombinant Antibody (clone Nacolomab); Fab Fragment | FC, IP, ELISA, Neut, FuncS, IF, ICC | Mouse Fab (IgG1, κ) |
| TAB-0388CL-F(E) | Mouse Anti-Human MUC1 Recombinant Antibody Fab Fragment | WB, IHC | |
| TAB-411MZ-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-411MZ-S(P)) | ELISA | Mouse scFv |
| PABX-183-S (P) | Recombinant Mouse Anti-MUC1 Antibody scFv Fragment (CTM01) | Neut, FuncS | scFv |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L036E | anti-MUC1 immunotoxin C242 (Fab)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L064E | anti-MUC1 immunotoxin H23 (scFv)-PE | Cytotoxicity assay, Functional assay |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0278CL-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-0278CL-S(P)) | ELISA, Internalization, BI, FuncS | Human scFv |
| TAB-431MZ-S(P) | Anti-Human MUC1 Recombinant Antibody scFv Fragment (PH1) | ELISA, WB, FC, IHC | Human antibody |
| TAB-432MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-432MZ-S(P)) | ELISA, FC | Human scFv |
| TAB-433MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-433MZ-S(P)) | ELISA, FC | Human scFv |
| TAB-434MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-434MZ-S(P)) | ELISA, FC | Human scFv |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-412MZ | Human Anti-MUC1 Recombinant Antibody (TAB-412MZ) | ELISA | Chimeric (mouse/human) IgG |
| TAB-412MZ-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-412MZ-S(P)) | ELISA | Mouse scFv |
| TAB-412MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-412MZ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-413MZ | Human Anti-MUC1 Recombinant Antibody (TAB-413MZ) | ELISA | Humanized IgG |
| TAB-425MZ | Anti-Human MUC1 Recombinant Antibody (huDMB5F3) | ELISA, FC, FuncS, ICC | Humanized antibody |
| TAB-413MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-413MZ-F(E)) | ELISA | Humanized Fab |
| TAB-425MZ-F(E) | Anti-Human MUC1 Recombinant Antibody Fab Fragment (huDMB5F3) | FC | Humanized antibody |
| TAB-430MZ-F(E) | Anti-Human MUC1 Recombinant Antibody Fab Fragment (BLC595) | ELISA, FC | Humanized antibody |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-012LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
| Gly-120LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
Sialylated IgG Glycan
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-012LC-1 | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-109LC | Recombinant Anti-Human MUC1 Antibody (Fc glycosylation) | ELISA | Human antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-121LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| Gly-122LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| Gly-123LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| Gly-124LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0379MZ | Chicken Anti-MUC1 Polyclonal IgY | IHC, WB | Chicken antibody |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC054 | PE-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC055 | APC-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC056 | BV421-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC096 | PE-A*02:01/Human MUC1 (LLLLTVLTV) MHC Tetramer | FCM | |
| MHC-YF272 | A*02:01/Human MUC1 (LLLLTVLTV) MHC Monomer | MHC Multimer |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2320 | Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB) | IHC-P, IHC-Fr | IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0838LC | Recombinant Mouse Anti-Human MUC1 Antibody (SM3) | ELISA | IgG1 |
| EPAF-0581CQ | Recombinant Mouse Anti-Human MUC1 Antibody (B27.29) | Neut, FC | IgG1 |
| EPAF-0583CQ | Recombinant Mouse Anti-Human MUC1 Antibody (BC4E549) | Neut, FC | IgG1 |
| EPAF-0584CQ | Recombinant Mouse Anti-Human MUC1 Antibody (DF3) | Neut, FC | IgG1 |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0091-YC | Mouse Anti-MUC1 Recombinant Antibody (HPAB-0091-YC) | ELISA, Neut | Mouse IgG |
| HPAB-0094-YC | Mouse Anti-MUC1 Recombinant Antibody (clone 12D10) | ELISA, FC | Mouse IgG2a |
| HPAB-0670-CN | Human Anti-MUC1 Recombinant Antibody (HPAB-0670-CN) | FC | Human IgG4, κ |
| HPAB-0272CQ | Human Anti-MUC1 Recombinant Antibody (HPAB-0272CQ) | ELISA, FC, FuncS | Human IgG1, κ |
| PABC-399 | Mouse Anti-MUC1 Recombinant Antibody (clone AR20.5) | ELISA, FC, FuncS | Mouse IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0091-YC-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-0091-YC-S(P)) | ELISA, Neut | Mouse scFv |
| HPAB-0495-FY-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-0495-FY-S(P)) | ELISA | Human scFv |
| HPAB-0496-FY-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-0496-FY-S(P)) | ELISA | Human scFv |
| HPAB-J0212-YC-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone BW835); scFv Fragment | ELISA, IHC | Mouse scFv |
| FAMAB-0211JF-S(P) | Human Anti-MUC1 Recombinant Antibody (clone G8); scFv Fragment | ELISA, FuncS | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H56 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Pemtumomab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-H65 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Sontuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-026ML | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Gatipotuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-166 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Cantuzumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| AFC-TAB-H77 | Afuco™ Human Anti-MUC1 Recombinant Antibody, ADCC Enhanced (AFC-TAB-H77) | ELISA, FC, IP, FuncS, IF | IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1159WJ-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone 12E); Fab Fragment | ELISA, ICC | Mouse Fab |
| HPAB-1160WJ-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone 3D); Fab Fragment | ELISA, ICC | Mouse Fab |
| HPAB-1161WJ-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone A5); Fab Fragment | ELISA, ICC | Mouse Fab |
| HPAB-1162WJ-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone C4); Fab Fragment | ELISA, ICC | Mouse Fab |
| HPAB-1163WJ-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone B5); Fab Fragment | ELISA, ICC | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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