Mouse Anti-MUC1 Recombinant Antibody (clone 12D10) (CAT#: HPAB-0094-YC)

Figure 1 shows an experimental result of examination with FACS of whether a MUC1 protein expressed on a cancer cell membrane surface binds to a 12D10 antibody or not.

The graphs show the result of analysis by FACS Aria. The left part indicates the result on a breast cancer cell T-47D, the center part indicates a human lung cancer cultured cell, Calu-3 cell, and the right part indicates the result on a mammary gland epithelial cell 184A which as a control. A dotted line indicates a control IgG, and a solid line indicates the 12D10 antibody. From this result, it was demonstrated that the 12D10 antibody strongly reacts with a breast cancer celland a lung cancer cell, but hardly reacts with a mammary gland epithelial cell

Figure 2 shows a standard curve for quantification of MUC1 by a sandwich immunoassay using the antibody 12D10.

Figure 1 Mouse Anti-MUC1 Recombinant Antibody (HPAB-0094-YC) in SDS-PAGE

SDS-PAGE analysis of HPAB-0094-YC in reduced (lane 1) and non-reduced (lane 2) conditions.

Figure 2 Mouse Anti-MUC1 Recombinant Antibody (HPAB-0094-YC) in HPLC

HPLC analysis of HPAB-0094-YC shows the purity is greater than 95%.

Figure 3 Mouse Anti-MUC1 Recombinant Antibody (HPAB-0094-YC) in WB

Western blot analysis of HPAB-0094-YC was performed with MUC1 Protein (his Tag).
HPAB-0094-YC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)

Figure 4 Anti-MUC1 Recombinant Antibody (HPAB-0094-YC) in WB

WB analysis of HPAB-0094-YC was performed by coating with Human MUC1 protein, His tag.