Human Anti-MUC1 (clone 1B2) scFv-Fc Chimera
CAT#: VS-0125-FY26
The anti-MUC1 single-chain antibody-Fc (scFv-Fc) product is a biotherapeutic targeted against the MUC1 glycoprotein. MUC1 is typically highly expressed in breast cancer, ovarian cancer, and several other malignancies, while its expression levels in normal tissues are relatively low, making it an important candidate target for cancer targeted therapy. By targeting MUC1, the anti-MUC1 scFv-Fc product can effectively recognize and bind to cancer cells, thereby inducing cell apoptosis or initiating an immune response. This targeted therapy is expected to enhance treatment efficacy, reduce the damage caused by traditional therapies to normal cells, and thereby improve patients' quality of life and survival rates.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg
Normal Tissue
Figure 5 Stomach
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (CAPAN-2, OE19, RPTEC TERT1, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- 1B2
- Applications
- Flow Cytometry
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The development of this antibody may bring innovative therapies for cancer treatment.
Target
- Alternative Names
- Mucin 1, Cell Surface Associated; Tumor-Associated Epithelial Membrane Antigen; Breast Carcinoma-Associated Antigen DF3; Peanut-Reactive Urinary Mucin; Polymorphic Epithelial Mucin; Carcinoma-Associated Mucin; Mucin 1, Transmembrane; Krebs Von Den Lungen-6; Cancer Antigen 15-3; Episialin; CA 15-3; H23AG; MUC-1; KL-6; PEMT; EMA; PUM; PEM
- Gene ID
- 4582
- UniProt ID
- P15941
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus, Secreted
- Post Translation Modifications
- Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
The N-terminal sequence has been shown to begin at position 24 or 28.
- Protein Refseq
- NP_001018016.1; NP_001018017.1; NP_001037855.1
- Function
- The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-AP504-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (HPAB-AP504-YC) | FC | Camel VHH |
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| HPAB-0736-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (HPAB-0736-YJ-VHH) | ELISA | Camelid VHH |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-166 | Humanized Anti-MUC1 Recombinant Antibody (clone Cantuzumab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | Humanized (from mouse) IgG1, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L036E | anti-MUC1 immunotoxin C242 (Fab)-PE | Cytotoxicity assay, Functional assay | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-412MZ | Human Anti-MUC1 Recombinant Antibody (TAB-412MZ) | ELISA | Chimeric (mouse/human) IgG |
| TAB-412MZ-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-412MZ-S(P)) | ELISA | Mouse scFv |
| TAB-412MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-412MZ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-431MZ-S(P) | Anti-Human MUC1 Recombinant Antibody scFv Fragment (PH1) | ELISA, WB, FC, IHC | Human antibody |
| TAB-432MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-432MZ-S(P)) | ELISA, FC | Human scFv |
| TAB-433MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-433MZ-S(P)) | ELISA, FC | Human scFv |
| TAB-434MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-434MZ-S(P)) | ELISA, FC | Human scFv |
| PABX-143-F (E) | Recombinant Human Anti-MUC1 Antibody Fab Fragment (CTM01) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-411MZ-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-411MZ-F(E)) | ELISA | Mouse Fab |
| TAB-416MZ-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-416MZ-F(E)) | IF | Mouse Fab |
| TAB-417MZ-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-417MZ-F(E)) | IF | Mouse Fab |
| TAB-418MZ-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-418MZ-F(E)) | ELISA, SDS-PAGE, IHC | Mouse Fab |
| TAB-419MZ-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-419MZ-F(E)) | ELISA, SDS-PAGE, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-012LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
| Gly-120LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-012LC-1 | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-109LC | Recombinant Anti-Human MUC1 Antibody (Fc glycosylation) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-121LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| Gly-122LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| Gly-124LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0379MZ | Chicken Anti-MUC1 Polyclonal IgY | IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC054 | PE-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC055 | APC-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC056 | BV421-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC096 | PE-A*02:01/Human MUC1 (LLLLTVLTV) MHC Tetramer | FCM | |
| MHC-YF272 | A*02:01/Human MUC1 (LLLLTVLTV) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2320 | Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0838LC | Recombinant Mouse Anti-Human MUC1 Antibody (SM3) | ELISA | IgG1 |
| EPAF-0581CQ | Recombinant Mouse Anti-Human MUC1 Antibody (B27.29) | Neut, FC | IgG1 |
| EPAF-0583CQ | Recombinant Mouse Anti-Human MUC1 Antibody (BC4E549) | Neut, FC | IgG1 |
| EPAF-0584CQ | Recombinant Mouse Anti-Human MUC1 Antibody (DF3) | Neut, FC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0091-YC-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0091-YC-F(E)) | ELISA, Neut | Mouse Fab |
| HPAB-0094-YC-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone 12D10); Fab Fragment | ELISA, FC | Mouse Fab |
| HPAB-J0212-YC-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone BW835); Fab Fragment | ELISA, IHC | Mouse Fab |
| HPAB-1159WJ-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone 12E); Fab Fragment | ELISA, ICC | Mouse Fab |
| HPAB-1160WJ-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone 3D); Fab Fragment | ELISA, ICC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0670-CN | Human Anti-MUC1 Recombinant Antibody (HPAB-0670-CN) | FC | Human IgG4, κ |
| ZG-0015C | Mouse Anti-MUC1 Recombinant Antibody (clone ABT-EMA) | IHC-P, IF | Mouse IgG |
| ZG-0016C | Mouse Anti-MUC1 Recombinant Antibody (clone PT0132) | IHC-P, IF | Mouse IgG |
| VS3-WK723 | Mouse Anti-MUC1 Recombinant Antibody (clone K72c8) | WB, ELISA | Mouse IgG |
| VS3-WK724 | Mouse Anti-MUC1 Recombinant Antibody (clone K73d9) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0872LY-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-0872LY-S(P)) | ELISA, WB, FC | Mouse scFv |
| HPAB-0873LY-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-0873LY-S(P)) | ELISA, WB, FC | Human scFv |
| HPAB-0874LY-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-0874LY-S(P)) | ELISA, WB, FC | Mouse scFv |
| HPAB-0875LY-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-0875LY-S(P)) | ELISA, WB, FC | Human scFv |
| FAMAB-0211JF-S(P) | Human Anti-MUC1 Recombinant Antibody (clone G8); scFv Fragment | ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H56 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H56) | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-H65 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H65) | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-026ML | Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-026ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-166 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-166) | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| AFC-TAB-H77 | Afuco™ Human Anti-MUC1 Recombinant Antibody, ADCC Enhanced (AFC-TAB-H77) | ELISA, FC, IP, FuncS, IF | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP745-YC | Mouse Anti-MUC1 Recombinant Antibody (HPAB-AP745-YC) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY192 | AbPlus™ Anti-MUC1 Magnetic Beads (139H2) | IP, Protein Purification | |
| VS-0724-YC1494 | AbPlus™ Anti-MUC1 Magnetic Beads (VS-0724-YC1494) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY169 | CytoStream™ Mouse Anti-MUC1 Recombinant Antibody (VS-0225-XY169) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1394 | Anti-MUC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4575 | Anti-Human MUC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4576 | Anti-Mouse MUC1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC379 | Recombinant Anti-MUC1 Vesicular Antibody, EV Displayed (VS-0425-YC379) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
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