Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated)
CAT#: Gly-012LC
This product is a humanized monoclonal antibody with N-glycosylation sites at CDR2 of VH. The glycosylation in CDR2 increased the half-life of the antibody. It specifically binds human MUC1 and can be potentially used in the treatment of ovarian cancer.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg
Normal Tissue
Figure 5 Stomach
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (CAPAN-2, OE19, RPTEC TERT1, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1
❮
❯
Glyco-engineering
- Glycosylation site
- CDR2 of VH
- Glycosylation Type
- N-glycosylation
- Activity
- Increased half-life
Specifications
- Target
- MUC1 (Mucin 1)
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized antibody
- Specificity
- Human
- Antibody Isotype
- IgG
- Clone
- hPM (humanized Pankomab)
- Expression Host
- GT-5s (Fuc+)
- Applications
- ELISA, FC, IHC
Target
- Alternative Names
- MUC1; mucin 1, cell surface associated; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-
- Related Disease
- Ovarian cancer
- Gene ID
- 4582
- UniProt ID
- P15941
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Dependable stability and storage
Storage and stability have been very reliable. We accidentally left the antibody at 4°C for a week, but it retained its activity, proving to be very robust.
Reduced immunogenic responses after modification
The glycosylation/sialylation modification truly makes a difference. We've seen improved performance in our in vivo studies with reduced immunogenic responses.
High Credibility in ELISA
The specificity of this antibody has greatly improved the reliability of our ELISA experiments. The glycosylation process ensures a stable and constant performance.
Q&As
-
Is it suitable for immunohistochemistry (IHC)?
A: Yes, this antibody has been validated for compatibility with IHC applications.
-
Can this antibody be used for flow cytometry?
A: Certainly. The antibody in question is adept for flow cytometry applications aimed at identifying MUC1's surface expression.
-
What species reactivity does this antibody have?
A: This antibody specifically reacts with human MUC1 protein.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone hPM (humanized Pankomab)"
See other products for "MUC1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2049-VHH | Recombinant Anti-human MUC1 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| HPAB-AP504-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (HPAB-AP504-YC) | FC | Camel VHH |
| HPAB-AP505-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (HPAB-AP505-YC) | ELISA | Camel VHH |
| HPAB-0736-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (HPAB-0736-YJ-VHH) | ELISA | Camelid VHH |
| HPAB-0737-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (HPAB-0737-YJ-VHH) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L036E | anti-MUC1 immunotoxin C242 (Fab)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L064E | anti-MUC1 immunotoxin H23 (scFv)-PE | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0278CL | Human Anti-MUC1 Recombinant Antibody (TAB-0278CL) | ELISA, Internalization, BI, FuncS | Human IgG |
| TAB-0278CL-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-0278CL-S(P)) | ELISA, Internalization, BI, FuncS | Human scFv |
| TAB-431MZ-F(E) | Anti-Human MUC1 Recombinant Antibody Fab Fragment (PH1) | ELISA, WB, FC, IHC | Human antibody |
| TAB-432MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-432MZ-F(E)) | ELISA, FC | Human Fab |
| PABX-143-S (P) | Recombinant Human Anti-MUC1 Antibody scFv Fragment (CTM01) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0388CL-S(P) | Mouse Anti-Human MUC1 Recombinant Antibody scFv Fragment | WB, IHC | |
| TAB-411MZ | Mouse Anti-MUC1 Recombinant Antibody (TAB-411MZ) | ELISA | Mouse IgG |
| TAB-437MZ | Mouse Anti-MUC1 Recombinant Antibody (TAB-437MZ) | FC, ELISA | Mouse IgG |
| TAB-438MZ | Mouse Anti-MUC1 Recombinant Antibody (TAB-438MZ) | FC, ELISA | Mouse IgG |
| TAB-439MZ | Anti-Human MUC1 Recombinant Antibody (1B2) | FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-412MZ | Human Anti-MUC1 Recombinant Antibody (TAB-412MZ) | ELISA | Chimeric (mouse/human) IgG |
| TAB-412MZ-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-412MZ-S(P)) | ELISA | Mouse scFv |
| TAB-412MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-412MZ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-413MZ | Human Anti-MUC1 Recombinant Antibody (TAB-413MZ) | ELISA | Humanized IgG |
| TAB-425MZ | Anti-Human MUC1 Recombinant Antibody (huDMB5F3) | ELISA, FC, FuncS, ICC | Humanized antibody |
| TAB-430MZ | Anti-Human MUC1 Recombinant Antibody (BLC595) | ELISA, FC | Humanized antibody |
| TAB-425MZ-S(P) | Anti-Human MUC1 Recombinant Antibody scFv Fragment (huDMB5F3) | FC | Humanized antibody |
| TAB-026ML | Anti-Human MUC1 Recombinant Antibody (TAB-026ML) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-012LC-1 | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-109LC | Recombinant Anti-Human MUC1 Antibody (Fc glycosylation) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-120LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-121LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| Gly-122LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| Gly-124LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0379MZ | Chicken Anti-MUC1 Polyclonal IgY | IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC054 | PE-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC055 | APC-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC056 | BV421-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
| MHC-LC781 | A*0201/Human MUC-1 (LLLLTVLTV) MHC Tetramer | FCM | |
| MHC-YF272 | A*02:01/Human MUC1 (LLLLTVLTV) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2320 | Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0838LC | Recombinant Mouse Anti-Human MUC1 Antibody (SM3) | ELISA | IgG1 |
| EPAF-0581CQ | Recombinant Mouse Anti-Human MUC1 Antibody (B27.29) | Neut, FC | IgG1 |
| EPAF-0583CQ | Recombinant Mouse Anti-Human MUC1 Antibody (BC4E549) | Neut, FC | IgG1 |
| EPAF-0584CQ | Recombinant Mouse Anti-Human MUC1 Antibody (DF3) | Neut, FC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0225YC | Mouse Anti-MUC1 Recombinant Antibody (clone MUSE11) | IHC | Mouse IgG1 |
| HPAB-0670-CN | Human Anti-MUC1 Recombinant Antibody (HPAB-0670-CN) | FC | Human IgG4, κ |
| HPAB-0872LY | Mouse Anti-MUC1 Recombinant Antibody (HPAB-0872LY) | ELISA, WB, FC | Mouse IgG1 |
| HPAB-0873LY | Human Anti-MUC1 Recombinant Antibody (HPAB-0873LY) | ELISA, WB, FC | Humanized IgG1 |
| HPAB-0874LY | Mouse Anti-MUC1 Recombinant Antibody (HPAB-0874LY) | ELISA, WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0872LY-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0872LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-0873LY-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0873LY-F(E)) | ELISA, WB, FC | Humanized Fab |
| HPAB-0874LY-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0874LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-0875LY-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0875LY-F(E)) | ELISA, WB, FC | Humanized Fab |
| HPAB-0207-YJ-F(E) | Human Anti-MUC1 Recombinant Antibody (clone Clivatuzumab (hPAM4)); Fab Fragment | ELISA, IHC, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H56 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H56) | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-H65 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H65) | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-026ML | Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-026ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-166 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-166) | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| AFC-TAB-H77 | Afuco™ Human Anti-MUC1 Recombinant Antibody, ADCC Enhanced (AFC-TAB-H77) | ELISA, FC, IP, FuncS, IF | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP745-YC | Mouse Anti-MUC1 Recombinant Antibody (HPAB-AP745-YC) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0212-YC-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone BW835); scFv Fragment | ELISA, IHC | Mouse scFv |
| HPAB-1159WJ-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone 12E); scFv Fragment | ELISA, ICC | Mouse scFv |
| HPAB-1160WJ-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone 3D); scFv Fragment | ELISA, ICC | Mouse scFv |
| HPAB-1161WJ-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone A5); scFv Fragment | ELISA, ICC | Mouse scFv |
| HPAB-1162WJ-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone C4); scFv Fragment | ELISA, ICC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY192 | AbPlus™ Anti-MUC1 Magnetic Beads (139H2) | IP, Protein Purification | |
| VS-0724-YC1494 | AbPlus™ Anti-MUC1 Magnetic Beads (VS-0724-YC1494) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY26 | Human Anti-MUC1 (clone 1B2) scFv-Fc Chimera | FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY169 | CytoStream™ Mouse Anti-MUC1 Recombinant Antibody (VS-0225-XY169) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1394 | Anti-MUC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4575 | Anti-Human MUC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4576 | Anti-Mouse MUC1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC379 | Recombinant Anti-MUC1 Vesicular Antibody, EV Displayed (VS-0425-YC379) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC131 | Recombinant Anti-MUC1 Biparatopic Antibody, Tandem scFv | ELISA, IHC | Tandem scFv |
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