Anti-Human PRNP Recombinant Antibody (V5B2)
CAT#: MRO-954LC
This product is a recombinant mouse anti-human prion protein monoclonal antibody. V5B2 specifically binds to PrP which is an infectious agent composed entirely of protein material and can be potentially used in the diagnosis and treatment of transmissible spongiform encephalopathies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Normal Tissue
Figure 4 Caudate
Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse antibody
- Specificity
- Human
- Clone
- V5B2
- Applications
- Enzyme-linked immunosorbent assay
- Related Disease
- Transmissible spongiform encephalopathies
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for MRO-954LC. Click the button above to contact us or submit your feedback about this product.
Excellent Sensitivity
I found that this antibody has excellent sensitivity in detecting endogenous PRNP levels. It's easy to use and gives consistent results in my studies.
Effective with Care
The antibody is effective but requires careful handling. Any deviation in protocol can affect results, so strict adherence is essential.
Good with Limitations
It's a good antibody overall and performs well in my ELISA tests, but I found it less effective in IHC, efficacy for other applications may need further exploration.
Great for Neuroscience Research
Perfect for my research in neuroscience. The antibody works well with brain tissue sections and has provided valuable insights into PRNP distribution.
Consistent Performance
I've used the antibody for several months now and it consistently performs well. It's been instrumental in my protein quantitation assays.
Q&As
-
What applications is the Anti-Human PRNP Recombinant Antibody (V5B2) suitable for?
A: The antibody is suitable for Enzyme-linked immunosorbent assay (ELISA).
-
What is the sensitivity of the antibody in ELISA?
A: This antibody performs well in our tests. And the sensitivity in ELISA generally high due to its recombinant nature.
-
Can this antibody be used in animal model studies?
A: It is primarily validated for human studies; compatibility with animal models should be tested independently.
-
How do I determine the optimal dilution for my experiment?
A: Perform a titration assay to determine the optimal dilution for your specific application.
-
What quality control tests are performed on this antibody?
A: The antibody undergoes rigorous quality control tests including specificity, sensitivity, and reproducibility.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone V5B2"
- CAT
- Product Name
See other products for "PRNP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B034(A) | Recombinant Anti-human PRNP Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | scFv-(D-Arg)9 |
| IAB-B034(G) | Recombinant Anti-human PRNP Intrabody [+36 GFP] | WB, CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B034(T) | Recombinant Anti-human PRNP Intrabody [Tat] | IF, ELISA, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-310 | Mouse Anti-PRNP Recombinant Antibody (clone POM2); Fab Fragment | WB, IHC, FuncS | Mouse Fab |
| FAMAB-0272CQ-F(E) | Human Anti-PRNP Recombinant Antibody (clone ch31C6); Fab Fragment | ELISA, WB, IHC | Chimeric (mouse/human) Fab |
| FAMAB-0273CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 106); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| FAMAB-0274CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 110); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| FAMAB-0275CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 132); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-026 | Recombinant Anti-Human PRNP VHH Single Domain Antibody (PNBL-026) | WB, ELISA, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-943LC | Anti-Mouse Prnp Recombinant Antibody (D13) | ELISA | Human antibody |
| MRO-944LC | Anti-Mouse Prnp Recombinant Antibody (D18) | ELISA | Human antibody |
| MRO-945LC | Anti-Mouse Prnp Recombinant Antibody (R72) | ELISA | Human antibody |
| MRO-946LC | Anti-Mouse Prnp Recombinant Antibody (R1) | ELISA | Human antibody |
| MRO-947LC | Anti-Mouse Prnp Recombinant Antibody (R2) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-953LC | Anti-Human PRNP Recombinant Antibody (1A1) | FuncS, FC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-942LC-F(E) | Anti-Sheep PRNP Recombinant Antibody Fab Fragment (6.1) | ELISA | Mouse antibody |
| MRO-948LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (6H10) | ELISA | Mouse antibody |
| MRO-949LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (31C6) | ELISA | Mouse antibody |
| MRO-950LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (72-5) | ELISA | Mouse antibody |
| MRO-951LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (44B1) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC, IF | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-161 | Mouse Anti-PRNP Recombinant Antibody (clone POM1) | ELISA, IP, IHC, FuncS | Mouse IgG1 |
| HPAB-0285CQ | Human Anti-PRNP Recombinant Antibody (clone boPrP-20) | ELISA, WB | Human IgG |
| PABC-254 | Mouse Anti-PRNP Recombinant Antibody (clone POM6) | ELISA, WB, IP | Mouse IgG |
| ZG-0346U | Rabbit Anti-PRNP Recombinant Antibody (clone T16-R) | ELISA, WB, IP | Rabbit IgG |
| ZG-0360U | Rabbit Anti-PRNP Recombinant Antibody (clone V21-V) | ELISA, WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0294-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone D18); scFv Fragment | WB, IF, IHC | Human scFv |
| FAMAB-0295-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone 3:3); scFv Fragment | WB, IF, IHC | Human scFv |
| FAMAB-0296-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone 6:4); scFv Fragment | WB, IF, IHC | Human scFv |
| FAMAB-0297-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone 6:6); scFv Fragment | WB, IF, IHC | Human scFv |
| HPAB-0282CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone boPrP-15); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY84 | Human Anti-PRNP (clone mAb132) scFv-Fc Chimera | ELISA, WB | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1763 | Anti-PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5727 | Anti-Human PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5728 | Anti-Mouse PRNP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC520 | Recombinant Anti-PRNP Vesicular Antibody, EV Displayed (VS-0425-YC520) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC150 | Recombinant Anti-PRNP (AA 56-90 x AA 83-89) Biparatopic Antibody, Tandem scFv (mAb 106 x mAb 110) | ELISA, IHC, WB | Tandem scFv |
| VS-0525-YC152 | Recombinant Anti-PRNP (AA 119-127 x AA 143-149) Biparatopic Antibody, Tandem scFv (mAb 132 x Clone 31C6) | ELISA, IHC, WB | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: ELISA, FuncS
Application: ELISA, FC, IF, IHC, WB
Application: WB, ELISA, IHC, IP, SPR, FuncS
Application: ELISA, PD, Activ, PK, Stim
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
