Z-domain of protein A scaffold protein anti-Human IL-6

Name: Z-domain of protein A scaffold protein anti-Human IL-6
SKU: SZA-L232
Rating: 5 (1 reviews)

CAT#: SZA-L232

This IL-6 specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=500 pM) for IL-6 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg

Normal Tissue Figure 4 Urinary bladder

Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg

Normal Tissue Figure 5 Lymph node

Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6

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Specifications

Scaffold Name
Z-domain of protein A

Origin
Staphylococcal protein A

Core Structure
3-α helixes

Variable Regions
13 Residues on first and second helix surface

Target
IL-6

Species Reactivity
Human

Expression Host
E. coli

Affinity Constant
500 pM

Applications
ELISA; IHC; Microscopy; FC; WB; FuncS

Target

Alternative Names
Staphylococcal protein A; Z-domain of protein A; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2

Gene ID
3569

UniProt ID
P05231

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Related Diseases

Inflammatory Bowel Diseases

Rheumatoid Arthritis

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Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

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Datasheet

Datasheet - SZA-L232.pdf

MSDS

MSDS - SZA-L232.pdf

COA

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