Mouse Anti-AKT2 Recombinant Antibody (clone 7F12-H0-B7)
CAT#: VS3-QX36
This product is a mouse antibody that recognizes AKT2. The antibody can be used in applications: WB, IHC, ICC, FC, ELISA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1304_E6_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity(granular pattern) in purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1518_H7_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1323_E6_2_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105221-AKT2
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Specifications
- Immunogen
- Purified recombinant fragment of human AKT2 expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Akt2
- Species Reactivity
- Human, Rat, Monkey
- Clone
- 7F12-H0-B7
- Applications
- WB, IHC, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC: 1:200-1:1000; ELISA: 1:10000
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Ras Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Prostate Cancer
Endocrine Resistance
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AKT2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-49-sdAb | Recombinant Anti-human AKT2 VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0033MZ | Chicken Anti-AKT2 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0128 | Hi-Affi™ Rabbit Anti-AKT2 Recombinant Antibody (clone DS128AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0383U | Rabbit Anti-AKT2 Recombinant Antibody (clone D16-H) | ELISA, WB, IP, ICC | Rabbit IgG |
| ZG-0384U | Rabbit Anti-AKT2 Recombinant Antibody (clone Q10-E) | ICC | Rabbit IgG |
| ZG-0430U | Rabbit Anti-AKT2 Recombinant Antibody (clone Y14-H), FITC | FC | Rabbit IgG |
| ZG-0431U | Rabbit Anti-AKT2 Recombinant Antibody (clone S7-R), FITC | FC | Rabbit IgG |
| VS3-CJ874 | Mouse Anti-AKT2 Recombinant Antibody (VS3-CJ874) | WB, ICC, IF, IHC, IP, ChIP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY16 | Mouse Anti-NHP AKT2 Recombinant Antibody (clone 1B6) | WB, IHC, ICC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY284 | Anti-AKT2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY285 | Anti-Mouse AKT2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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