Mouse Anti-BRAF Recombinant Antibody (ZG-053R) (CAT#: ZG-053R)

Name: Mouse Anti-BRAF Recombinant Antibody (ZG-053R)
SKU: ZG-053R
Rating: 5 (1 reviews)

This product is a mouse antibody that recognizes BRAF. It can be used in a wide variety of applications, such as: WB, ELISA, IHC.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Mouse Anti-BRAF Antibody (ZG-053R) in WB

Figure 1 Mouse Anti-BRAF Antibody (ZG-053R) in WB

Western Blot analysis using Raf-B monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRYBraf cDNA (2).

IHC

Figure 2 Mouse Anti-BRAF Antibody (ZG-053R) in IHC

Figure 2 Mouse Anti-BRAF Antibody (ZG-053R) in IHC

Immunohistochemistry analysis of paraffin-embedded human bladder carcinoma tissue (left) and lung carcinoma tissue (right) showing cytoplasmic localization with DAB staining using Raf-B monoclonal antibody.

Subcellular Location

Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg

Normal Tissue

Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg

Figure 3 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg

Figure 4 Cerebral cortex
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg

Figure 5 Colon
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg

Figure 6 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg

Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg

Figure 8 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg

Figure 9 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg

RNA Expression

Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Purified recombinant fragment of Raf-B expressed in E. coli

Host Species
Mouse

Type
Mouse IgG

Species Reactivity
Human

Applications
WB, ELISA, IHC

Product Property

Purification
Affinity purified

Purity
>95%

Format
Liquid

Concentration
1 mg/mL

Storage
Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
WB: 1:500-1:2000, IHC: 1:200-1:1000, ELISA: 1:10000

Target

Alternative Names
B-RAF1; B-raf1; NS7; RAFB1; BRAF

Gene ID
673

UniProt ID
P15056

Related Resources

Related Signaling Pathways
Related Diseases

cAMP Signaling Pathway

MAPK Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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MOB-122	Recombinant Anti-human BRAF Antibody	ELISA, WB, Dot, FuncS	IgG
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CAT	Product Name	Application
TAB-1284CL	Anti-Human BRAF Recombinant Antibody (CCTCC C2012122)	IB, IF
TAB-1284CL-S(P)	Anti-Human BRAF Recombinant Antibody scFv Fragment (CCTCC C2012122)	IB, IF
TAB-1284CL-F(E)	Anti-Human BRAF Recombinant Antibody Fab Fragment (CCTCC C2012122)	IB, IF

CAT	Product Name	Application
MHC-CN0457	PE-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer	FCM, IHC
MHC-CN0458	APC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer	FCM, IHC
MHC-CN0459	FITC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer	FCM, IHC
MHC-YF341	B*27:05/Human BRAF (600E) (GRFGLATEK) MHC Pentamer	FCM
MHC-YF342	B*27:05/Human BRAF (600V) (GRFGLATVK) MHC Pentamer	FCM

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