Rabbit Anti-BRAF Recombinant Antibody (ZG-0571J) (CAT#: ZG-0571J)
This product is a rabbit antibody that recognizes BRAF.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-BRAF Antibody (ZG-0571J) in WB
Western Blot
Positive WB detected in A549 whole cell lysate(treated with EGF or not)
All lanes Phospho-BRAF antibody at 1.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 KDa
Observed band size: 85 KDa
IF
Figure 2 Rabbit Anti-BRAF Antibody (ZG-0571J) in IF
Immunofluorescence staining of HepG2 cells with ZG-0571J at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-BRAF (T401)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BRAF
- Species Reactivity
- Human
- Applications
- ELISA, WB, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- NS7; B-raf; BRAF1; RAFB1; B-RAF1; serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; murine sarcoma viral (v-raf) oncogene homolog B1; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1
- Gene ID
- 673
- UniProt ID
- P15056
Related Resources
cAMP Signaling Pathway
MAPK Signaling Pathway
Throid Cancer
Cushing Syndrome
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BRAF"
Select a product category from the dropdown menu below to view related products.
Please select product type
Fab Antibody Products
ScFv Antibody Products
Mouse Antibody Products
IgG Antibody Products
Rabbit Monoclonal Antibody Products
MHC Tetramer Products for Cancer
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-F(E) | Recombinant Anti-human BRAF Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| MHH-122-F(E) | Recombinant Human Anti-human BRAF Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0571J. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: WB, ELISA, FuncS
-2.png)
Application: WB, ELISA
Application: ELISA, IHC, FC, WB, IF, IA
Application: WB, IHC, ELISA, FC
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, BL
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, Inhib, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
