What is the clone name and isotype of this antibody?

This is the recombinant mouse antibody clone C775. It has a Mouse IgG1, κ (kappa) isotype. The antibody reacts with a specific epitope of Human CCL2 and is suitable for use in ELISA assays.

What is the function of the CCL2 protein?

CCL2 (C-C Motif Chemokine Ligand 2), also known as MCP-1, is a chemokine that attracts monocytes, memory T cells, and dendritic cells to sites of inflammation produced by either tissue injury or infection. It is a protein coding gene affiliated with the lncRNA class.

Mouse Anti-CCL2 Recombinant Antibody (clone C775)

CAT#: HPAB-1658-FY

This product is a recombinant antibody that recognizes CCL2. The monoclonal antibody C775 reacts with the epitope of CCL2.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg

Subcellular Location Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg

Subcellular Location Figure 3 IHC staining of human appendix

Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg

Subcellular Location Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg

Normal Tissue Figure 6 Colon

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg

Normal Tissue Figure 7 Kidney

Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2

❮

❯

Specifications

Host Species
Mouse

Type
Mouse IgG1, κ

Specificity
Human CCL2

Species Reactivity
Human

Clone
C775

Applications
ELISA

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
C-C Motif Chemokine Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE; Small Inducible Cytokine A2 (Monocyte Chemotactic Protein 1, Homologous To Mouse Sig-Je); Small Inducible Cytokine Subfamily A (Cys-Cys), Member 2; Monocyte Chemoattractant Protein-1; Chemokine (C-C Motif) Ligand 2; Monocyte Chemotactic Protein 1; Small-Inducible Cytokine A2; SCYA2; MCP-1

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for HPAB-1658-FY. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Sensitive Ccl2 ELISA Results

The C775 clone worked perfectly for our ELISA applications detecting Human CCL2. We needed an antibody that reacts with a specific epitope of the chemokine, and this recombinant product delivered. The consistency between batches has been helpful for our long-term study on monocyte chemotactic proteins.

Validated By HPLC Analysis

We were impressed by the quality control of this antibody, which is validated by both SDS-PAGE and HPLC analysis to be over 95% pure. It specifically recognizes the C-C Motif Chemokine Ligand 2, and we have successfully used it to analyze CCL2 levels in our research samples without any issues.

Q&As

What is the clone name and isotype of this antibody?

A: This is the recombinant mouse antibody clone C775. It has a Mouse IgG1, κ (kappa) isotype. The antibody reacts with a specific epitope of Human CCL2 and is suitable for use in ELISA assays.
What is the function of the CCL2 protein?

A: CCL2 (C-C Motif Chemokine Ligand 2), also known as MCP-1, is a chemokine that attracts monocytes, memory T cells, and dendritic cells to sites of inflammation produced by either tissue injury or infection. It is a protein coding gene affiliated with the lncRNA class.

View the frequently asked questions answered by Creative Biolabs Support.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Related Diseases

Rheumatoid Arthritis

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-1658-FY.pdf

MSDS

MSDS - HPAB-1658-FY.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "Clone C775"

CAT
Product Name

See other products for "CCL2"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1273z	Mouse Anti-CCL2 Recombinant Antibody (clone 15A10)	ELISA, WB	Mouse IgG1

CAT	Product Name	Application	Type
NABG-038	Recombinant Anti-Human CCL2 VHH Single Domain Antibody	WB, ELISA, IHC, FuncS	Llama VHH

CAT	Product Name	Application	Type
TAB-117	Anti-Human MCP-1 Recombinant Antibody (Carlumab)	ELISA, FC, IP, FuncS, IF, Neut, ICC	IgG1 - kappa

CAT	Product Name	Application	Type
PABL-421	Human Anti-CCL2 Recombinant Antibody (clone CNTO 888)	Inhib, IB, FuncS	Human IgG

CAT	Product Name	Application	Type
PFBL-418	Human Anti-CCL2 Recombinant Antibody (clone CNTO 888); Fab Fragment	WB, ELISA, FuncS	Human Fab

CAT	Product Name	Application	Type
PSBL-418	Human Anti-CCL2 Recombinant Antibody (clone CNTO 888); scFv Fragment	WB, ELISA, FuncS	Human scFv

CAT	Product Name	Application	Type
TAB-0744CL	Anti-Human CCL2 Recombinant Antibody (AAV293)	ELISA

CAT	Product Name	Application	Type
TAB-0745CL	Anti-Human CCL2 Recombinant Antibody (AAV294)	ELISA

CAT	Product Name	Application	Type
TAB-0748CL	Anti-Human CCL2 Recombinant Antibody (1.1.1)	ELISA	Human antibody

CAT	Product Name	Application	Type
TAB-0749CL	Anti-Human CCL2 Recombinant Antibody (1.10.1)	ELISA	Human antibody

CAT	Product Name	Application	Type
TAB-0750CL	Anti-Human CCL2 Recombinant Antibody (MC32)	ELISA, Block, Inhib	Human antibody

CAT	Product Name	Application	Type
TAB-0751CL	Anti-Human CCL2 Recombinant Antibody (MC8)	ELISA	Human antibody

CAT	Product Name	Application	Type
TAB-0754CL	Mouse Anti-CCL2 Recombinant Antibody (TAB-0754CL)	ELISA, SPR, DB, IHC	Mouse IgG1

CAT	Product Name	Application	Type
TAB-0755CL	Mouse Anti-CCL2 Recombinant Antibody (TAB-0755CL)	SPR, DB, IHC	Mouse IgG1

CAT	Product Name	Application	Type
TAB-0756CL	Mouse Anti-CCL2 Recombinant Antibody (TAB-0756CL)	ELISA, SPR, DB, IHC	Mouse IgG2b

CAT	Product Name	Application	Type
MOB-1149CT	Mouse Anti-CCL2 Recombinant Antibody (clone 2D8)	ELISA, IHC-P, sELISA	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-147CQ	Mouse Anti-CCL2 Recombinant Antibody (clone CBL605)	WB, ELISA(Cap), ICC, Neut	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-148CQ	Mouse Anti-CCL2 Recombinant Antibody (clone CBL221)	ELISA, WB, Neut	Mouse IgG1, κ

CAT	Product Name	Application	Type
NEUT-149CQ	Mouse Anti-CCL2 Recombinant Antibody (clone CBL436)	WB, Neut	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-150CQ	Mouse Anti-CCL2 Recombinant Antibody (clone CBL728)	WB, ELISA(Cap), Neut	Mouse IgG2b

CAT	Product Name	Application	Type
NEUT-151CQ	Mouse Anti-CCL2 Recombinant Antibody (clone MNA1)	WB, Neut, ELISA, FC, IA, IF, IHC, IHC-Fr, IHC-P, IP	Mouse IgG1

CAT	Product Name	Application	Type
MOR-0504	Hi-Affi™ Rabbit Anti-CCL2 Recombinant Antibody (clone DS504AB)	WB, IF	Rabbit IgG

CAT	Product Name	Application	Type
MOR-4543	Hi-Affi™ Rabbit Anti-CCL2 Recombinant Antibody (clone TH52DS)	WB	Rabbit IgG

CAT	Product Name	Application	Type
AFC-TAB-117	Afuco™ Anti-CCL2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-117)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-1658-FY-F(E)	Mouse Anti-CCL2 Recombinant Antibody (clone C775); scFv Fragment	ELISA	Mouse scFv

CAT	Product Name	Application	Type
HPAB-1658-FY-S(P)	Mouse Anti-CCL2 Recombinant Antibody (clone C775); Fab Fragment	ELISA	Mouse Fab, κ

CAT	Product Name	Application	Type
ZG-0273C	Mouse Anti-CCL2 Recombinant Antibody (ZG-0273C)	WB, ELISA	Mouse IgG

CAT	Product Name	Application	Type
ZG-0153U	Mouse Anti-CCL2 Recombinant Antibody (clone 3D2E5)	ELISA, IHC	Mouse IgG2b

CAT	Product Name	Application	Type
VS-0424-XY44	AbPlus™ Anti-CCL2 Magnetic Beads (MNA1)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-1024-XY319	Mouse Anti-NHP CCL2 Recombinant Antibody (clone 2D8)	WB, IHC, IF, FC, ELISA	Mouse IgG1

CAT	Product Name	Application	Type
VS-0225-XY9	CytoStream™ Armenian Hamster Anti-CCL2 Recombinant Antibody (clone 2H5)	ELISA, FC, Neut	Armenian Hamster IgG1, kappa

CAT	Product Name	Application	Type
VS-0225-XY10	CytoStream™ Mouse Anti-CCL2 Recombinant Antibody (clone 5D3-F7)	FC	Mouse IgG1, kappa

CAT	Product Name	Application	Type
VS13-YC144	CytoStream™ Rabbit Anti-CCL2 Polyclonal Antibody (VS13-YC144)	WB, ELISA, IHC-P, IHC-Fr, FC, IF	Rabbit IgG

CAT	Product Name	Application	Type
VS-0425-FY151	Mouse Anti-CCL2 scFv-Fc Chimera (VS-0425-FY151)	ELISA, IHC, WB	Mouse IgG2b, scFv-Fc

CAT	Product Name	Application	Type
VS-0525-XY1055	Anti-CCL2 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1056	Anti-Mouse CCL2 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0625-YC45	Recombinant Anti-CCL2 Eliminating Antibody, pH-Sensitive (VS-0625-YC45)	Antigen-Sweeping In Vivo.	Human IgG1 kappa

CAT	Product Name	Application	Type
VS-0825-YC30	SmartAb™ Recombinant Anti-CCL2 pH-dependent Antibody (Clone C775)	ELISA	Mouse IgG1 kappa

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Anti-IL22 Recombinant Antibody (TAB-748) in HPLC

Anti-IL22 Recombinant Antibody (TAB-748) (CAT# TAB-748)
Application: WB, IP, IF, FuncS, FC, Neut, ELISA

Figure 4 Anti-Human CSF2 Recombinant Antibody (TAB-H44) in SEC-HPLC

Anti-Human CSF2 Recombinant Antibody (TAB-H44) (CAT# TAB-H44)
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC

Figure 6 Anti-Human VEGF Recombinant Antibody (TAB-011) in ELISA

Anti-Human VEGF Recombinant Antibody (Bevacizumab) (CAT# TAB-011)
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC

Figure 2 Anti-Human IL6R Antibody (TAB-026) in WB

Anti-Human IL6R Recombinant Antibody (TAB-026) (CAT# TAB-026)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Figure 2 Anti-Human SLAMF7 Recombinant Antibody (TAB-570CL) in ELISA

Human Anti-SLAMF7 Recombinant Antibody (TAB-570CL) (CAT# TAB-570CL)
Application: IF, IHC, Inhib

Figure 1 Anti-histone Antibody-Cytokine Fusion Protein, IgG-IL12 (ACFP-SH113) in ELISA

Anti-histone Antibody-Cytokine Fusion Protein, IgG (NHS76)-IL12 (CAT# ACFP-SH113)
Application:

Figure 2 Mouse Anti-DOTA Recombinant Antibody (PABW-133) in HPLC

Mouse Anti-DOTA Recombinant Antibody (clone 2D12.5) (CAT# PABW-133)
Application: ELISA

Figure 4 Anti-Human CEACAM5 Recombinant Antibody (PABL-496) in ELISA

Recombinant Mouse Anti-HCV E2 Antibody (AP33) (CAT# PABL-496)
Application: FC, Neut, FuncS

Figure 2 Recombinant Anti-VEEV E2 Antibody (PABL-742) in HPLC

Recombinant Human Anti-VEEV E2 Antibody (3B4C-4) (CAT# PABL-742)
Application: Neut

Figure 1 Mouse Anti-α4β7 Recombinant Antibody (PABL-746) in ELISA

Mouse Anti-α4β7 Recombinant Antibody (PABL-746) (CAT# PABL-746)
Application: FC

Figure 3 Anti-Human PERP Antibody (TAB-1017CL) in Flow cytometry

Anti-Human PERP Recombinant Antibody (KM3411) (CAT# TAB-1017CL)
Application: FC

Figure 2 Anti-Human SIRPA Recombinant Antibody Fab Fragment (TAB-453CT-F(E)) in HPLC

Human Anti-SIRPA Recombinant Antibody; Fab Fragment (TAB-453CT-F(E)) (CAT# TAB-453CT-F(E))
Application: WB, ELISA

Figure 2 Anti-Human CCP Recombinant Antibody (TAB-393CQ) in HPLC

Anti-Human CCP Recombinant Antibody (A02) (CAT# TAB-393CQ)
Application: ELISA, IHC

Figure 3 Anti-Human SNCA Recombinant Antibody (TAB-0741CLV) in DB

Mouse Anti-SNCA Recombinant Antibody (TAB-0741CLV) (CAT# TAB-0741CLV)
Application: IF

Figure 3 Human Anti-APLNR Recombinant Antibody (TAB-0938CLV) in FACS

Human Anti-APLNR Recombinant Antibody (TAB-0938CLV) (CAT# TAB-0938CLV)
Application: ELISA

Figure 1 Mouse Anti-ROR1 Recombinant Antibody (HPAB-0308-CN) in SDS-PAGE

Mouse Anti-ROR1 Recombinant Antibody (HPAB-0308-CN) (CAT# HPAB-0308-CN)
Application: ELISA, FC

Figure 3 Anti-LAM Recombinant Antibody (HPAB-M0560-YC) in ELISA

Human Anti-LAM Recombinant Antibody (HPAB-M0560-YC) (CAT# HPAB-M0560-YC)
Application: ELISA, WB, Microarray, Block

Figure 2 Human Anti-FRβ Recombinant Antibody (m909) (HPAB-0792-FY) in ELISA.

Human Anti-FRβ Recombinant Antibody (HPAB-0792-FY) (CAT# HPAB-0792-FY)
Application: ELISA

Figure 2 Human Anti-Insulin Recombinant Antibody (clone mAb48) (FAMAB-0224WJ) in ELISA

Human Anti-Insulin Recombinant Antibody (clone mAb48) (CAT# FAMAB-0224WJ)
Application: ELISA, WB

Figure 4 Human Anti-FZD5 Recombinant Antibody (PABJ-0145) in ELISA

Human Anti-FZD5 Recombinant Antibody (PABJ-0145) (CAT# PABJ-0145)
Application: ELISA, Inhib, FuncS

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.