CytoStream™ Rabbit Anti-CCL2 Polyclonal Antibody (VS13-YC144)
CAT#: VS13-YC144
The CytoStream™ Rabbit Anti-CCL2 Polyclonal Antibody is specific for human and rat CCL2. CCL2 encodes a chemokine with chemotactic activity, attracting monocytes and influencing conditions with monocytic infiltrates, involved in receptor interactions and pathogenesis of conditions like atherosclerosis. The anti-CCL2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
Subcellular Location
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
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Specifications
- Immunogen
- Recombinant human MCP-1 (aa 24-99) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CCL2
- Species Reactivity
- Human, Rat, Mouse (Predicted), Dog (Predicted), Pig (Predicted), Horse (Predicted), Rabbit (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunofluorescence (IF): 1/100-1/500
Target
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Related Diseases
Rheumatoid Arthritis
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CCL2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-038 | Recombinant Anti-Human CCL2 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-418 | Human Anti-CCL2 Recombinant Antibody (clone CNTO 888); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| HPAB-1658-FY-S(P) | Mouse Anti-CCL2 Recombinant Antibody (clone C775); Fab Fragment | ELISA | Mouse Fab, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-418 | Human Anti-CCL2 Recombinant Antibody (clone CNTO 888); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| HPAB-1658-FY-F(E) | Mouse Anti-CCL2 Recombinant Antibody (clone C775); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0744CL | Anti-Human CCL2 Recombinant Antibody (AAV293) | ELISA | |
| TAB-0745CL | Anti-Human CCL2 Recombinant Antibody (AAV294) | ELISA | |
| TAB-0754CL | Mouse Anti-CCL2 Recombinant Antibody (TAB-0754CL) | ELISA, SPR, DB, IHC | Mouse IgG1 |
| TAB-0755CL | Mouse Anti-CCL2 Recombinant Antibody (TAB-0755CL) | SPR, DB, IHC | Mouse IgG1 |
| TAB-0756CL | Mouse Anti-CCL2 Recombinant Antibody (TAB-0756CL) | ELISA, SPR, DB, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0748CL | Anti-Human CCL2 Recombinant Antibody (1.1.1) | ELISA | Human antibody |
| TAB-0749CL | Anti-Human CCL2 Recombinant Antibody (1.10.1) | ELISA | Human antibody |
| TAB-0750CL | Anti-Human CCL2 Recombinant Antibody (MC32) | ELISA, Block, Inhib | Human antibody |
| TAB-0751CL | Anti-Human CCL2 Recombinant Antibody (MC8) | ELISA | Human antibody |
| TAB-0752CL | Anti-Human CCL2 Recombinant Antibody (MC15) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-150CQ | Mouse Anti-CCL2 Recombinant Antibody (clone CBL728) | WB, ELISA(Cap), Neut | Mouse IgG2b |
| NEUT-151CQ | Mouse Anti-CCL2 Recombinant Antibody (clone MNA1) | WB, Neut, ELISA, FC, IA, IF, IHC, IHC-Fr, IHC-P, IP | Mouse IgG1 |
| NEUT-152CQ | Mouse Anti-CCL2 Recombinant Antibody (clone S8) | ELISA, Neut | Mouse IgG1, κ |
| NEUT-153CQ | Mouse Anti-CCL2 Recombinant Antibody (NEUT-153CQ) | Neut | Mouse IgG1 |
| NEUT-154CQ | Mouse Anti-CCL2 Recombinant Antibody (clone S101) | ELISA, Neut, WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0504 | Hi-Affi™ Rabbit Anti-CCL2 Recombinant Antibody (clone DS504AB) | WB, IF | Rabbit IgG |
| MOR-4543 | Hi-Affi™ Rabbit Anti-CCL2 Recombinant Antibody (clone TH52DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-117 | Afuco™ Anti-CCL2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-117) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY177 | Mouse Anti-CCL2 Recombinant Antibody (clone 1A7B8) | ELISA, WB | Mouse IgG1 |
| VS3-WK1674 | Recombinant Mouse Anti-CCL2 Capture Antibody (clone 4A2) | ELISA | Mouse IgG2b |
| VS3-WK1675 | Recombinant Mouse Anti-CCL2 Detection Antibody (clone 6E1) | ELISA | Mouse IgG2b |
| VS3-WK1688 | Recombinant Rabbit Anti-CCL2 Capture Antibody (clone R08-5X2) | ELISA | Rabbit IgG |
| VS3-WK1689 | Recombinant Rabbit Anti-CCL2 Detection Antibody (clone R08-2Y4) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY44 | AbPlus™ Anti-CCL2 Magnetic Beads (MNA1) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY319 | Mouse Anti-NHP CCL2 Recombinant Antibody (clone 2D8) | WB, IHC, IF, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY9 | CytoStream™ Armenian Hamster Anti-CCL2 Recombinant Antibody (clone 2H5) | ELISA, FC, Neut | Armenian Hamster IgG1, kappa |
| VS-0225-XY10 | CytoStream™ Mouse Anti-CCL2 Recombinant Antibody (clone 5D3-F7) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY151 | Mouse Anti-CCL2 scFv-Fc Chimera (VS-0425-FY151) | ELISA, IHC, WB | Mouse IgG2b, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1055 | Anti-CCL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1056 | Anti-Mouse CCL2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC45 | Recombinant Anti-CCL2 Eliminating Antibody, pH-Sensitive (VS-0625-YC45) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
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