Human Anti-CCL2 Recombinant Antibody (clone CNTO 888)
CAT#: PABL-421
Recombinant Human Antibody (CNTO 888) is capable of binding to CCL2, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-CCL2 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-CCL2 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody binds CCL2 with high affinity (KD = 22 pM) and inhibits CCL2 binding to its receptor and consequentfunctional activity.
Published Data
Gene Expression
Inhib
Figure 1 CNTO888 inhibits PC-3ᴸᵘᶜ cell proliferation and migration in vitro.
PC-3ᴸᵘᶜ cells were stimulated with CCL2 (10 or 100 ng/mL) for 72 h in the presence or absence of CNTO888 (30 μg/mL), C1142 (30 μg/mL), or CNTO888 + C1142 (30 μg/mL each). The control antibody (Ab) for CNTO888 is anti-huIgG and the control antibody for C1142 is C1322 (a rat/mouse chimeric nonspecific antibody). Cell viability was measured by WST-1. Columns, mean percentage of serum-free medium control; bars, SD (*, P < 0.05; **, P < 0.01). SFM, serum-free medium.
Loberg, R. D., Ying, C., Craig, M., Day, L. L., Sargent, E., Neeley, C., ... & Pienta, K. J. (2007). Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo. Cancer research, 67(19), 9417-9424.
IB
Figure 2 immunoblot analysis of Akt, p70S6 kinase, and mitogen-activated protein kinase p44/p42 activation.
Representative immunoblots from four independent experiments. 1, control; 2,CCL2 (100 ng/mL, 24 h); 3, CCL2(100 ng/mL) + CNTO888 (30 μg/mL); 4, CNTO888(30 μg/mL).
Loberg, R. D., Ying, C., Craig, M., Day, L. L., Sargent, E., Neeley, C., ... & Pienta, K. J. (2007). Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo. Cancer research, 67(19), 9417-9424.
Inhib
Figure 3 Efficacy of single-agent anti-CCL2 antibodies compared with single-agent docetaxel in vivo.
Inhibition of CCL2 was compared with docetaxel (MTD 40 mg/kg) by bioluminescent imaging and quantification of total tumor burden. Mice received PC-3ᴸᵘᶜ cells by intracardiac injection (n = 10 per group). Beginning on day 14, mice received PBS (□), (CNTO888 (●), C1142),CNTO888 + C1142 (*), or docetaxel ( △ ) by i.p. injection (arrow, when treatment began). Solid lines, human antibodies; dashed lines, mouse antibodies.
Loberg, R. D., Ying, C., Craig, M., Day, L. L., Sargent, E., Neeley, C., ... & Pienta, K. J. (2007). Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo. Cancer research, 67(19), 9417-9424.
FuncS
Figure 4 Anti-CCL2 antibodies in combination with docetaxel induce tumor regression in vivo.
Inhibition of CCL2 in combination with docetaxel (MTD 40 mg/kg)was assessed by bioluminescent imaging and quantification of total tumor burden and compared with docetaxel alone. Mice received PC-3Luccells by intracardiacinjection. Beginning on day 14, mice received PBS (□), docetaxel + CNTO888 (●), docetaxel + C1142 (□), docetaxel + CNTO888 + C1142 (*), or docetaxel ( w ) by i.p.injection. Docetaxel was administered every week for 3 wks (weeks 2–4), whereas antibodies were delivered twice per week for 6 wks (weeks 2–8). On week 8, allantibody treatments were stopped and tumor progression was monitored to week 12. [arrow (1), initiation of treatment; arrow (2), cessation of docetaxel; arrow (3),cessation of antibody therapy].
Loberg, R. D., Ying, C., Craig, M., Day, L. L., Sargent, E., Neeley, C., ... & Pienta, K. J. (2007). Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo. Cancer research, 67(19), 9417-9424.
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Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
Subcellular Location
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
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Specifications
- Immunogen
- Human chemokine (C-C motif) ligand 2
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- CCL2
- Species Reactivity
- Human
- Clone
- CNTO 888
- Applications
- Inhib, IB, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Inhibition, Immunoblotting and Function Assay.
Target
- Alternative Names
- CCL2; chemokine (C-C motif) ligand 2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je);
- Gene ID
- 6347
- UniProt ID
- P13500
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Related Diseases
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Related Signaling Pathways
TNF Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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