Human Anti-CCL2 Recombinant Antibody (clone CNTO 888)
CAT#: PABL-421
Recombinant Human Antibody (CNTO 888) is capable of binding to CCL2, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-CCL2 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-CCL2 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody binds CCL2 with high affinity (KD = 22 pM) and inhibits CCL2 binding to its receptor and consequentfunctional activity.
Published Data
Gene Expression
Inhib
Figure 1 CNTO888 inhibits PC-3ᴸᵘᶜ cell proliferation and migration in vitro.
PC-3ᴸᵘᶜ cells were stimulated with CCL2 (10 or 100 ng/mL) for 72 h in the presence or absence of CNTO888 (30 μg/mL), C1142 (30 μg/mL), or CNTO888 + C1142 (30 μg/mL each). The control antibody (Ab) for CNTO888 is anti-huIgG and the control antibody for C1142 is C1322 (a rat/mouse chimeric nonspecific antibody). Cell viability was measured by WST-1. Columns, mean percentage of serum-free medium control; bars, SD (*, P < 0.05; **, P < 0.01). SFM, serum-free medium.
Loberg, R. D., Ying, C., Craig, M., Day, L. L., Sargent, E., Neeley, C., ... & Pienta, K. J. (2007). Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo. Cancer research, 67(19), 9417-9424.
IB
Figure 2 immunoblot analysis of Akt, p70S6 kinase, and mitogen-activated protein kinase p44/p42 activation.
Representative immunoblots from four independent experiments. 1, control; 2,CCL2 (100 ng/mL, 24 h); 3, CCL2(100 ng/mL) + CNTO888 (30 μg/mL); 4, CNTO888(30 μg/mL).
Loberg, R. D., Ying, C., Craig, M., Day, L. L., Sargent, E., Neeley, C., ... & Pienta, K. J. (2007). Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo. Cancer research, 67(19), 9417-9424.
Inhib
Figure 3 Efficacy of single-agent anti-CCL2 antibodies compared with single-agent docetaxel in vivo.
Inhibition of CCL2 was compared with docetaxel (MTD 40 mg/kg) by bioluminescent imaging and quantification of total tumor burden. Mice received PC-3ᴸᵘᶜ cells by intracardiac injection (n = 10 per group). Beginning on day 14, mice received PBS (□), (CNTO888 (●), C1142),CNTO888 + C1142 (*), or docetaxel ( △ ) by i.p. injection (arrow, when treatment began). Solid lines, human antibodies; dashed lines, mouse antibodies.
Loberg, R. D., Ying, C., Craig, M., Day, L. L., Sargent, E., Neeley, C., ... & Pienta, K. J. (2007). Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo. Cancer research, 67(19), 9417-9424.
FuncS
Figure 4 Anti-CCL2 antibodies in combination with docetaxel induce tumor regression in vivo.
Inhibition of CCL2 in combination with docetaxel (MTD 40 mg/kg)was assessed by bioluminescent imaging and quantification of total tumor burden and compared with docetaxel alone. Mice received PC-3Luccells by intracardiacinjection. Beginning on day 14, mice received PBS (□), docetaxel + CNTO888 (●), docetaxel + C1142 (□), docetaxel + CNTO888 + C1142 (*), or docetaxel ( w ) by i.p.injection. Docetaxel was administered every week for 3 wks (weeks 2–4), whereas antibodies were delivered twice per week for 6 wks (weeks 2–8). On week 8, allantibody treatments were stopped and tumor progression was monitored to week 12. [arrow (1), initiation of treatment; arrow (2), cessation of docetaxel; arrow (3),cessation of antibody therapy].
Loberg, R. D., Ying, C., Craig, M., Day, L. L., Sargent, E., Neeley, C., ... & Pienta, K. J. (2007). Targeting CCL2 with systemic delivery of neutralizing antibodies induces prostate cancer tumor regression in vivo. Cancer research, 67(19), 9417-9424.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
Subcellular Location
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
❮
❯
Specifications
- Immunogen
- Human chemokine (C-C motif) ligand 2
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- CCL2
- Species Reactivity
- Human
- Clone
- CNTO 888
- Applications
- Inhib, IB, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Inhibition, Immunoblotting and Function Assay.
Target
- Alternative Names
- CCL2; chemokine (C-C motif) ligand 2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je);
- Gene ID
- 6347
- UniProt ID
- P13500
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABL-421. Click the button above to contact us or submit your feedback about this product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-421, RRID: AB_3714180)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Rheumatoid Arthritis
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone CNTO 888"
- CAT
- Product Name
See other products for "CCL2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1273z | Mouse Anti-CCL2 Recombinant Antibody (clone 15A10) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-038 | Recombinant Anti-Human CCL2 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-117 | Anti-Human MCP-1 Recombinant Antibody (Carlumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-418 | Human Anti-CCL2 Recombinant Antibody (clone CNTO 888); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-418 | Human Anti-CCL2 Recombinant Antibody (clone CNTO 888); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0744CL | Anti-Human CCL2 Recombinant Antibody (AAV293) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0745CL | Anti-Human CCL2 Recombinant Antibody (AAV294) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0748CL | Anti-Human CCL2 Recombinant Antibody (1.1.1) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0749CL | Anti-Human CCL2 Recombinant Antibody (1.10.1) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0750CL | Anti-Human CCL2 Recombinant Antibody (MC32) | ELISA, Block, Inhib | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0751CL | Anti-Human CCL2 Recombinant Antibody (MC8) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0754CL | Mouse Anti-CCL2 Recombinant Antibody (TAB-0754CL) | ELISA, SPR, DB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0755CL | Mouse Anti-CCL2 Recombinant Antibody (TAB-0755CL) | SPR, DB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0756CL | Mouse Anti-CCL2 Recombinant Antibody (TAB-0756CL) | ELISA, SPR, DB, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1149CT | Mouse Anti-CCL2 Recombinant Antibody (clone 2D8) | ELISA, IHC-P, sELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-147CQ | Mouse Anti-CCL2 Recombinant Antibody (clone CBL605) | WB, ELISA(Cap), ICC, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-148CQ | Mouse Anti-CCL2 Recombinant Antibody (clone CBL221) | ELISA, WB, Neut | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-149CQ | Mouse Anti-CCL2 Recombinant Antibody (clone CBL436) | WB, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-150CQ | Mouse Anti-CCL2 Recombinant Antibody (clone CBL728) | WB, ELISA(Cap), Neut | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-151CQ | Mouse Anti-CCL2 Recombinant Antibody (clone MNA1) | WB, Neut, ELISA, FC, IA, IF, IHC, IHC-Fr, IHC-P, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0504 | Hi-Affi™ Rabbit Anti-CCL2 Recombinant Antibody (clone DS504AB) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4543 | Hi-Affi™ Rabbit Anti-CCL2 Recombinant Antibody (clone TH52DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-117 | Afuco™ Anti-CCL2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-117) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1658-FY | Mouse Anti-CCL2 Recombinant Antibody (clone C775) | ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1658-FY-F(E) | Mouse Anti-CCL2 Recombinant Antibody (clone C775); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1658-FY-S(P) | Mouse Anti-CCL2 Recombinant Antibody (clone C775); Fab Fragment | ELISA | Mouse Fab, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0273C | Mouse Anti-CCL2 Recombinant Antibody (ZG-0273C) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0153U | Mouse Anti-CCL2 Recombinant Antibody (clone 3D2E5) | ELISA, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY44 | AbPlus™ Anti-CCL2 Magnetic Beads (MNA1) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY319 | Mouse Anti-NHP CCL2 Recombinant Antibody (clone 2D8) | WB, IHC, IF, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY9 | CytoStream™ Armenian Hamster Anti-CCL2 Recombinant Antibody (clone 2H5) | ELISA, FC, Neut | Armenian Hamster IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY10 | CytoStream™ Mouse Anti-CCL2 Recombinant Antibody (clone 5D3-F7) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC144 | CytoStream™ Rabbit Anti-CCL2 Polyclonal Antibody (VS13-YC144) | WB, ELISA, IHC-P, IHC-Fr, FC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY151 | Mouse Anti-CCL2 scFv-Fc Chimera (VS-0425-FY151) | ELISA, IHC, WB | Mouse IgG2b, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1055 | Anti-CCL2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1056 | Anti-Mouse CCL2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC45 | Recombinant Anti-CCL2 Eliminating Antibody, pH-Sensitive (VS-0625-YC45) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC30 | SmartAb™ Recombinant Anti-CCL2 pH-dependent Antibody (Clone C775) | ELISA | Mouse IgG1 kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, Inhib, ICC, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
