AbPlus™ Anti-CD44 Magnetic Beads (1M7.8.1)

Name: AbPlus™ Anti-CD44 Magnetic Beads (1M7.8.1)
SKU: VS-0424-XY53
Rating: 5 (1 reviews)

CAT#: VS-0424-XY53

The AbPlus Anti-CD44 Magnetic Beads (1M7.8.1) is an innovative affinity resin which is bound with anti-CD44 specific antibody (1M7.8.1). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of CD44 protein under native and denaturing conditions.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg

Subcellular Location Figure 3 IHC staining of human vagina

Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg

Normal Tissue Figure 4 Colon

Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg

Normal Tissue Figure 6 Skin

Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg

Normal Tissue Figure 7 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44

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Specifications

Applications
Immunoprecipitation, Protein Purification

Matrix
Magrose bead (> 50 μmol/mL gel)

Bead Ligand
Anti-CD44 specific antibody (1M7.8.1)

Target
CD44

Immunogen
Mouse 80-95 kD lymphocyte surface glycoprotein CD44

Target Species
Human, Mouse, Rat

Antibody Clone
1M7.8.1

Bead Capacity
20-30 mg/mL binding antibody

Bead size
10-37 μm

Stability
pH 2-14

Format
20% Suspension

Buffer
PBS, pH 7.4, with 1% BSA

Preservative
0.03% Proclin 300

Storage
Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.

Applications

Application Notes
The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Target

Introduction
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Alternative Names
CD44; CD44 molecule (Indian blood group); IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system

Gene ID
960

UniProt ID
P16070

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Datasheet

Datasheet - VS-0424-XY53.pdf

MSDS

MSDS - VS-0424-XY53.pdf

COA

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