Rat Anti-CD44 Recombinant Antibody (clone 9A4); scFv Fragment (CAT#: HPAB-N0328-YC-S(P))
The antibody (9A4) is a rat monoclonal antibody to CD44, variant 6. This antibody reacts with mouse. The CD44, variant 6 Antibody (9A4) has been validated for the following applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg
Figure 3 IHC staining of human vagina
(Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg
(Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg
Figure 4 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg
Figure 6 Skin
(Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg
(Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
Specifications
- Host Species
- Rat
- Type
- Rat scFv
- Specificity
- Human CD44
- Species Reactivity
- Human, Mouse
- Clone
- 9A4
- Applications
- WB, ELISA, IHC-P, IHC-Fr
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
- Gene ID
- 960
- UniProt ID
- P16070
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-991 | Mouse Anti-CD44 Recombinant Antibody (clone BU52) | IHC, WB, IP, Inhib, Stim | Mouse IgG1 F(ab')2, κ |
| HPAB-N0328-YC-F(E) | Rat Anti-CD44 Recombinant Antibody (clone 9A4); Fab Fragment | WB, ELISA, IHC-P, IHC-Fr | Rat Fab |
| HPAB-0141CQ-F(E) | Mouse Anti-CD44 Recombinant Antibody (clone A3D8); Fab Fragment | FC, IHC, WB | Mouse Fab |
| HPAB-1055-FY-S(P) | Mouse Anti-CD44 Recombinant Antibody; scFv Fragment (HPAB-1055-FY-S(P)) | IHC, Inhib, FC | Mouse scFv |
| HPAB-0443-YJ-F(E) | Human Anti-CD44 Recombinant Antibody (clone CA14); Fab Fragment | ELISA, FuncS | Human Fab |
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