Human Anti-CD44 (clone RG7356) scFv-Fc Chimera
CAT#: VS-0125-FY25
The anti-CD44 single-chain antibody-Fc (scFv-Fc) construct is a molecular design that combines a single-chain antibody (scFv) with the Fc region (typically derived from human immunoglobulin G, IgG). This design aims to enhance the stability of the antibody, prolong its circulation half-life, and increase its affinity for the target. CD44 is a glycoprotein widely expressed on the surface of various cell types and is involved in multiple biological processes such as cell adhesion, migration, and proliferation. Given that CD44 is expressed in a variety of tumor cells, the anti-CD44 scFv-Fc construct has the potential to serve as a multifunctional PET imaging agent for the diagnosis and monitoring of different types of CD44-positive tumors. Future clinical studies will help validate its efficacy and safety in tumor imaging, promoting its application in precision medicine for cancer.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human vagina
Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg
Normal Tissue
Figure 6 Skin
Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- RG7356
- Applications
- Bioimaging
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The potential function of anti-CD44 scFv-Fc lies in its ability to selectively target and visualize CD44-positive tumor cells. Given that CD44 is a glycoprotein frequently overexpressed in various cancers and cancer stem cells, this targeting could facilitate the imaging of these malignancies. Additionally, the interaction of CD44 with hyaluronic acid (HA) has implications for the activation of several cell signaling pathways that may contribute to enhanced cell proliferation, survival, and invasion. Thus, targeting CD44 presents a promising strategy for cancer therapy.
Target
- Alternative Names
- CD44 Molecule (Indian Blood Group); Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein 1; Hyaluronate Receptor; Hermes Antigen; CD44 Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3
- Gene ID
- 960
- UniProt ID
- P16070
- Cellular Localization
- Cell membrane, Cell projection, Membrane
- Post Translation Modifications
- Proteolytically cleaved in the extracellular matrix by specific proteinases in several cell lines and tumors.
N-glycosylated.
O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
- Protein Refseq
- NP_000601.3; NP_001001389.1; NP_001001390.1
- Function
- Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment.
Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection.
Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases.
Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
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- Product Name
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- CAT
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