Recombinant Mouse Antibody binds selectively to Mouse CD44.
Figure 1 CD44-expressing mesenchymal cells are found throughout newly formed fibrotic lung tissue obtained from patients who died from the fibroproliferative disorder of acute alveolar fibrosis after lung injury.
Lung tissue obtained immediately after death was snap frozen and immunostained with anti–CD44 monoclonal antibody.
Svee, K., White, J., Vaillant, P., Jessurun, J., Roongta, U., Krumwiede, M., ... & Henke, C. (1996). Acute lung injury fibroblast migration and invasion of a fibrin matrix is mediated by CD44. The Journal of clinical investigation, 98(8), 1713-1727.
Figure 2 Identification of the 85-kD fibroblast CD44 isoform by Western analysis with anti–CD44 monoclonal antibodies.
Western analysis of undigested and chondroitinase-digested acute lung injury fibroblast lysates.
Svee, K., White, J., Vaillant, P., Jessurun, J., Roongta, U., Krumwiede, M., ... & Henke, C. (1996). Acute lung injury fibroblast migration and invasion of a fibrin matrix is mediated by CD44. The Journal of clinical investigation, 98(8), 1713-1727.
Figure 3 Identification of the 85-kD fibroblast CD44 isoform by immunoprecipitation with anti–CD44 monoclonal antibodies.
Undigested and chondroitinase-digested Hermes 3 immunoprecipitated proteins from acute lung injury fibroblasts. Undigested and chondroitinase-digested proteins immunoprecipitated with monoclonal antibodies that only recognize the unsaturated bonds of either 4- or 6-sulfated chondroitin sulfate residues that remain attached to the core protein after chondroitinase digestion.
Svee, K., White, J., Vaillant, P., Jessurun, J., Roongta, U., Krumwiede, M., ... & Henke, C. (1996). Acute lung injury fibroblast migration and invasion of a fibrin matrix is mediated by CD44. The Journal of clinical investigation, 98(8), 1713-1727.
Figure 4 Anti-CD44 antibody inhibits acute lung injury fibroblast invasion of a fibrin matrix.
Acute lung injury fibroblasts were allowed to attach and spread (4 h, 378C) on the surface of tissue culture wells. They were then overlayed with a fibrin gel and cultured in the presence of anti–CD44 monoclonal antibody in varying concentrations: 0.01 mg/ml, 1.0 mg/ml, 20 mg/ml, or an isotype IgG1 control monoclonal antibody.
Svee, K., White, J., Vaillant, P., Jessurun, J., Roongta, U., Krumwiede, M., ... & Henke, C. (1996). Acute lung injury fibroblast migration and invasion of a fibrin matrix is mediated by CD44. The Journal of clinical investigation, 98(8), 1713-1727.
Figure 5 Fibroblast migration on the provisional matrix proteins fibronectin, fibrinogen are blocked by anti-CD44 antibody.
Acute lung injury fibroblasts were preincubated for 30 min with varying concentrations of antiCD44 antibody. Fibroblast migration to the undersides of filters coated with fibronectin was assessed using modified Boyden chambers in the presence of anti–CD44 antibody or IgG1 isotypematched control antibody.
Svee, K., White, J., Vaillant, P., Jessurun, J., Roongta, U., Krumwiede, M., ... & Henke, C. (1996). Acute lung injury fibroblast migration and invasion of a fibrin matrix is mediated by CD44. The Journal of clinical investigation, 98(8), 1713-1727.
Figure 6 Illustrates the effect of anti-CD44 mAb on CD2 receptor-stimulated T cell proliferation.
Figure 7 Illustrates the effects of anti-CD44 mAb on IL-2 production by T cells stimulated with OKT3
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-727 | Anti-CD44 Recombinant Antibody (Bivatuzumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 |
TAB-236LC | Anti-Human CD44 Recombinant Antibody (huPTA-4621) | FuncS | Humanized antibody |
TAB-236LC-S(P) | Anti-Human CD44 Recombinant Antibody scFv Fragment (huPTA-4621) | FC | Humanized antibody |
TAB-236LC-F(E) | Anti-Human CD44 Recombinant Antibody Fab Fragment (huPTA-4621) | FC | Humanized antibody |
There are currently no Customer reviews or questions for DrMAB-991. Click the button above to contact us or submit your feedback about this product.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.