Human Anti-CD44 Recombinant Antibody (clone (hu)ARH460-16-2 variant HV2/KV1) (CAT#: HPAB-0154-FY)

Recombinant monoclonal antibody to CD44. This product is a recombinant humanized antibody that recognizes human CD44.


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WB

Figure 1 Western blot of 500 micrograms of MDA-MB-231 membrane proteins probed with different primary antibody solutions.

Figure 1 Western blot of 500 micrograms of MDA-MB-231 membrane proteins probed with different primary antibody solutions.

Lanes 1-5 were probed with biotinylated H460-16-2 mixed with 2 micrograms/mL, 10 micrograms/mL, 100 micrograms/mL, 500 micrograms/mL or 1000 micrograms/mL of non-biotinylated H460-16-2 respectively. Lanes 6-10 were probed with biotinylated H460-16-2 mixed with 2 micrograms/mL, 10 micrograms/mL, 100 micrograms/mL, 500 micrograms/mL or 1000 micrograms/mL of non-biotinylated AR37A335.8 respectively. Lanes 11-15 were probed with biotinylated H460-16-2 mixed with 2 micrograms/mL, 10 micrograms/mL, 100 micrograms/mL, 500 micrograms/mL or 1000 micrograms/mL of non-biotinylated 1B7.11 respectively.

WB

Figure 2 Western blot of 500 micrograms of MDA-MB-231 membrane proteins probed with different primary antibody solutions.

Figure 2 Western blot of 500 micrograms of MDA-MB-231 membrane proteins probed with different primary antibody solutions.

Lanes 1-5 were probed with biotinylated AR37A335.8 mixed with 2 micrograms/mL, 10 micrograms/mL, 100 micrograms/mL, 500 micrograms/mL or 1000 micrograms/mL of non-biotinylated H460-16-2 respectively. Lanes 6-10 were probed with biotinylated AR37A335.8 mixed with 2 micrograms/mL, 10 micrograms/mL, 100 micrograms/mL, 500 micrograms/mL or 1000 micrograms/mL of non-biotinylated AR37A335.8 respectively. Lanes 11-15 were probed with biotinylated AR37A335.8 mixed with 2 micrograms/mL, 10 micrograms/mL, 100 micrograms/mL, 500 micrograms/mL or 1000 micrograms/mL of non-biotinylated 8B1B.1 respectively.


Specifications

  • Host Species
  • Human
  • Derivation
  • Humanized
  • Type
  • Humanized IgG1
  • Specificity
  • Human CD44
  • Species Reactivity
  • Human
  • Clone
  • (hu)ARH460-16-2 variant HV2/KV1
  • Applications
  • Inhib, WB
  • Related Disease
  • Cancer

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The CD44 antibody has been reported in applications of Inhib, WB.

Target

  • Alternative Names
  • CD44 Molecule (Indian Blood Group); Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein 1; Hyaluronate Receptor; Hermes Antigen; CD44 Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Chicken IgY Antibody

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BRD-0101MZ Chicken Anti-CD44 Polyclonal IgY WB Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-378CQ Rat Anti-CD44 Recombinant Antibody (clone 1M7.8.1) FC, IF, Inhib, IP, WB Rat IgG2b
NEUT-381CQ Human Anti-CD44 Recombinant Antibody (clone Hermes-3) ELISA, FC, IF, IHC, IP, Neut, WB Human IgG1, κ
NEUT-382CQ Rat Anti-CD44 Recombinant Antibody (clone IM7) Block Rat IgG2b
NEUT-383CQ Rat Anti-CD44 Recombinant Antibody (clone KM201), FITC Block, FC, IP, WB, FuncS Rat IgG1, κ
NEUT-384CQ Rat Anti-CD44 Recombinant Antibody (clone KM81) IF, IHC-P, FC, Block Rat IgG2a

Neutralizing Antibody

CAT Product Name Application Type
NEUT-379CQ Mouse Anti-CD44 Recombinant Antibody (clone F10-44-2) WB, Neut, FC, IHC, IHC-Fr, IHC-P, IP, ICC Mouse IgG2a
NEUT-380CQ Rat Anti-CD44 Recombinant Antibody (clone Hermes-1) WB, Neut, ELISA, FC, IF, IHC, IP Rat IgG2a

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0563 Hi-Affi™ Rabbit Anti-CD44 Recombinant Antibody (clone DS563AB) FC, IF, IHC-Fr, IHC-P, WB Rabbit IgG
MOR-4548 Hi-Affi™ Rabbit Anti-CD44 Recombinant Antibody (clone TH58DS) IF, ICC, FC Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-727 Afuco™ Anti-CD44 ADCC Recombinant Antibody (Bivatuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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