Anti-Mouse CD44 Immunohistochemistry Kit

Name: Anti-Mouse CD44 Immunohistochemistry Kit
SKU: VS-0525-XY1186
Rating: 5 (1 reviews)

CAT#: VS-0525-XY1186

Our CD44 IHC kit delivers comprehensive reagents for high-specificity staining of this important cell surface glycoprotein. Applicable across species and tissue types, the kit works on both paraffin-embedded and frozen samples. Its user-friendly protocol supports consistent performance in studies of cell adhesion and migration.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg

Subcellular Location Figure 3 IHC staining of human vagina

Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg

Normal Tissue Figure 4 Colon

Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg

Normal Tissue Figure 6 Skin

Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg

Normal Tissue Figure 7 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44

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Specifications

Application
IHC

Size
50 Tests

Species Reactivity
Human, Mouse

Target
CD44

Primary Antibody
Rabbit Anti-CD44 Antibody

Secondary Antibody
Goat anti-Rabbit Antibody, HRP

Sample Type
FFPE tissue; Frozen section tissue

Kit Storage
All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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Downloadable Resources

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Datasheet

Datasheet - VS-0525-XY1186.pdf
IHC Kits-FFPE Protocol.pdf
IHC Kits-FS Protocol.pdf

MSDS

MSDS - VS-0525-XY1186.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
TAB-231LC	Human Anti-CD44 Recombinant Antibody (TAB-231LC)	ELISA	Chimeric antibody (mouse/human)
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TAB-235LC	Anti-Human CD44 Recombinant Antibody (chPTA-4621)	FuncS	Chimeric antibody (mouse/human)
TAB-231LC-F(E)	Human Anti-CD44 Recombinant Antibody; Fab Fragment (TAB-231LC-F(E))	ELISA	Chimeric (mouse/human) Fab
TAB-232LC-F(E)	Human Anti-CD44 Recombinant Antibody; Fab Fragment (TAB-232LC-F(E))	ELISA	Chimeric (mouse/human) Fab

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TAB-237LC-F(E)	Anti-Human CD44 Recombinant Antibody Fab Fragment (LGadh01-01)	ELISA
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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HPAB-0563-WJ-F(E)	Human Anti-CD44 Recombinant Antibody (clone 1A9); Fab Fragment	ELISA, FC	Human Fab
HPAB-0564-WJ-F(E)	Human Anti-CD44 Recombinant Antibody (clone 2D1); Fab Fragment	ELISA, FC	Human Fab
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