SmartAb™ Recombinant Anti-CD86 pH-dependent Antibody (Clone Hu3D1)
CAT#: VS-0825-YC71
The anti-CD86 pH-dependent antibody (clone Hu3D1) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human B7-2. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for CD86 under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from CD86 at pH 6.0. This pH-sensitive antibody retains its binding affinity for CD86 on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. Human B7-2, also known as CD86, is a co-stimulatory molecule expressed on antigen-presenting cells. It provides the second signal for T-cell activation by binding to CD28 and CTLA-4, and its interaction with CD28 is crucial for the initiation of the adaptive immune response.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HDLM-2
Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26802/1962_A12_6_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4319/11663_B_8_5.jpg
Normal Tissue
Figure 3 Lung
Macrophages Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4319/11663_A_1_4.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4319/11663_A_8_3.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4319/11663_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114013-CD86
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Specifications
- Host Species
- Human
- Type
- Human IgG2/G4
- Specificity
- Human B7-2
- Species Reactivity
- Human
- Clone
- Hu3D1
- Applications
- ELISA
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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