anti-CD86 immunotoxin 1G10 (IgG)-Bouganin

Name: anti-CD86 immunotoxin 1G10 (IgG)-Bouganin
SKU: AGTO-L002O
Rating: 5 (1 reviews)

CAT#: AGTO-L002O

This immunotoxin was achieved by conjugating/fusing the anti-CD86 monoclonal antibody to Bouganin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to CD86-expressing cells. Once bind, the toxin enters cells by receptor-mediated endocytosis and enzymatically damages ribosomes, ultimately leading to cell death. It was designed for treatment of Hodgkin's disease or other CD86-expressing tumours.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HDLM-2

Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane & centriolar satellites.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26802/1962_A12_6_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4319/11663_B_8_5.jpg

Normal Tissue Figure 3 Lung

Macrophages Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4319/11663_A_1_4.jpg

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4319/11663_A_8_3.jpg

Normal Tissue Figure 5 Lymph node

Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4319/11663_A_9_8.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114013-CD86

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Specifications

Host Species
Rat

Species Reactivity
Mouse

Clone
1G10

Molecule Class
L kappa; IgG2a-Bouganin

Toxin Description
Bouganin is a type-I ribosome inactivating protein (RIP) that was recently purified from leaves of Bougainvillea spectabilis Willd. Like all type-I RIPs, bouganin is a RNA glycosidase with deadenylating activity. Bouganin was characterized as a single-chain protein with a molecular mass of 26 200 Da and its mature protein consists of 250 amino acids. Furthermore, it was found that bouganin is probably the least toxic RIP known based on protein inhibiting studies in cell lines. It was observed that the bouganin concentrations inhibiting protein synthesis of human cell lines were much higher than the concentrations required to inhibit protein synthesis in a rabbit reticulocyte lysate assay, a characteristic common to all type-I RIPs.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
Hodgkin's lymphoma

Target

Alternative Names
CD86; CD86 molecule; B70; B7-2; B7.2; LAB72; CD28LG2; T-lymphocyte activation antigen CD86; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); Hodgkin's lymphoma; Bouganin

Gene ID
942

UniProt ID
A5D8T4

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

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Datasheet

Datasheet - AGTO-L002O.pdf

MSDS

MSDS - AGTO-L002O.pdf

COA

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