Human Anti-DLL4 Recombinant Antibody (clone YW26.82) (CAT#: HPAB-0156CQ)
This product is a recombinant human antibody that recognizes DLL4. Mab YW26.82 selectively binds to mouse and human DLL4. It recognized a binding determinant present in EGF-like repeat number 2 (EGL2) of the human DLL4 extracellular domain (ECD). EGL2 comprises amino acids 252-282 of the human DLL4 ECD.
Specific Inquiry
IHC
Figure 1 DLL4-mediated Notch signaling regulated EC pro liferation.
Anti-DLL4 antibody (YW26.82) or DBZ promoted the sprouting of HUVECs
ELISA
Figure 2 Characterization of anti-DLL4 antibody.
The binding of YW26.82 at indicated concentrations was measured by ELISA assay. Bound antibodies were detected with anti-human antibody HRP conjugate using TMB as substrate and OD 450 nm absorbance measurement. Anti-HER2 and recombinant ErbB2-ECD were used as assay control.
FC
Figure 3 FACS analysis of 293 cells transiently transfected with vector, full length DLL4, Jag1 or DLL1. Significant binding of YW26.82 was only detected on DLL4 transfected cells.
Expression of Jag1 and DLL1 was con firmed by the binding of recombinant rat Notch1-Fc and recombinant rat Notch2-Fc, respectively. YW26.82. rrNotch1-Fc or rrNotch2-Fc were used at 2 ug/ml followed by goat anti-human IgG-PE.
Block
Figure 4 Anti-DLL4 antibody blocked the binding of DLL4-AP, but not DLL1-AP, to coated rNotch1.
Specifications
- Host Species
- Human
- Type
- Human IgG1, κ
- Specificity
- Human DLL4
- Species Reactivity
- Human, Mouse
- Clone
- YW26.82
- Applications
- ELISA, FC, IHC, Block
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Flow Cytometry and Blocking. Please see the Published Data for further information.
Target
- Alternative Names
- Delta Like Canonical Notch Ligand 4; Drosophila Delta Homolog 4; Delta4; Delta-Like 4 Homolog (Drosophila); Delta-Like 4 (Drosophila); Delta-Like 4 Homolog; Delta-Like 4 Protein; Delta-Like Protein 4;
- Gene ID
- 54567
- UniProt ID
- Q9NR61
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Humanized Antibody
Rat Antibody
Chimeric Antibody
Human Antibody
Mouse Antibody
Blocking Antibody
Rabbit Monoclonal Antibody
Recombinant Antibody
Fab Fragment Antibody
scFv Fragment Antibody
ADCC Enhanced Antibody
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-204 | Anti-Human DLL4 Recombinant Antibody (Demcizumab) | ELISA, FC, IP, FuncS, IF, Neut, WB | IgG2 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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