Human Anti-DLL4 Recombinant Antibody (HPAB-0156CQ) (CAT#: HPAB-0156CQ)
This product is a recombinant human antibody that recognizes DLL4. This antibody selectively binds to mouse and human DLL4. It recognized a binding determinant present in EGF-like repeat number 2 (EGL2) of the human DLL4 extracellular domain (ECD). EGL2 comprises amino acids 252-282 of the human DLL4 ECD.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_2_selected.jpg
(Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_2_selected.jpg
Figure 2 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_9_3.jpg
Figure 3 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_4.jpg
Figure 4 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_6.jpg
Figure 6 RNA cell line category: Group enriched (BJ hTERT+, HUVEC TERT2, SCLC-21H)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128917-DLL4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128917-DLL4
Specifications
- Host Species
- Human
- Type
- Human IgG1, κ
- Specificity
- Human DLL4
- Species Reactivity
- Human, Mouse
- Applications
- ELISA, FC, IHC, Block
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Flow Cytometry and Blocking. Please see the Published Data for further information.
Target
- Alternative Names
- Delta Like Canonical Notch Ligand 4; Drosophila Delta Homolog 4; Delta4; Delta-Like 4 Homolog (Drosophila); Delta-Like 4 (Drosophila); Delta-Like 4 Homolog; Delta-Like 4 Protein; Delta-Like Protein 4;
- Gene ID
- 54567
- UniProt ID
- Q9NR61
Related Resources
Park, Mi Ae, et al. "Activation of the arterial program drives development of definitive hemogenic endothelium with lymphoid potential." Cell reports 23.8 (2018): 2467-2481. https://doi.org/10.1016/j.celrep.2018.04.092
This research investigates how activating the arterial program drives the development of definitive hemogenic endothelium with lymphoid potential from human pluripotent stem cells (hPSCs). The study demonstrates that enhancing arterial specification through ETS1 overexpression or modulation of MAPK/ERK signaling pathways at the mesodermal stage of development dramatically improves the formation of arterial-type hemogenic endothelium (AHE) with a specific phenotype (CD144+CD43-CD73-DLL4+CXCR4+/-). Blood cells generated from this arterial hemogenic endothelium showed more than 100-fold enrichment in T cell precursor frequency and possessed the capacity to produce B lymphocytes and red blood cells expressing high levels of BCL11a and β-globin, indicating definitive hematopoiesis with lymphoid potential.
Creative Biolabs provided DLL4 neutralizing antibodies that were instrumental in confirming that the effect of ETS1 upregulation on hematopoiesis is mediated through NOTCH signaling. When these antibodies were used to block DLL4, they effectively abrogated the enhancing effect of ETS1 on blood production and colony-forming cell potential, demonstrating that DLL4 expression and NOTCH activation are essential for ETS1-mediated promotion of the definitive hematoendothelial program. This contribution helped establish the molecular pathway linking arterial specification to lymphoid potential in hPSC-derived hematopoietic cells.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-204 | Anti-Human DLL4 Recombinant Antibody (TAB-204) | ELISA, FC, IP, FuncS, IF, Neut, WB | IgG2 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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