Human Anti-F8 Recombinant Antibody (clone DH10) (CAT#: FAMAB-0038JF)
Recombinant monoclonal antibody can specially bind to human factor VIII. This human anti-F8 antibody DH10 can interfere with factor VIII function, so it can be wildly used in the treatment of pateints with hemophilia A.
Specific Inquiry
WB
Figure 1 WB analysis of purified F8 in transgenic N. benthamiana plants.
Stained gel with lanes corresponding to (M) marker proteins and the indicated F8 proteins is shown.
Tavladoraki, P., Benvenuto, E., Trinca, S., De Martinis, D., Cattaneo, A., & Galeffi, P. (1993). Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature, 366(6454), 469-472.
ELISA
Figure 2 ELISA analysis of purified F8 in transgenic N. benthamiana plants.
The data are presented in OD at 415 nm.
Tavladoraki, P., Benvenuto, E., Trinca, S., De Martinis, D., Cattaneo, A., & Galeffi, P. (1993). Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature, 366(6454), 469-472.
IF
Figure 3 Immunofluorescence analysis performed on F9 murine teratocarcinoma.
Scale bar=50 mm.
Villa, A., Trachsel, E., Kaspar, M., Schliemann, C., Sommavilla, R., Rybak, J. N., ... & Neri, D. (2008). A high‐affinity human monoclonal antibody specific to the alternatively spliced EDA domain of fibronectin efficiently targets tumor neo‐vasculature in vivo. International journal of cancer, 122(11), 2405-2413.
Dot
Figure 4 Dot Blot analysis of the supernatants of 96 induced cultures of individual library clones.
70% of the clones expressed a detectable amount of soluble scFv fragment.
Villa, A., Trachsel, E., Kaspar, M., Schliemann, C., Sommavilla, R., Rybak, J. N., ... & Neri, D. (2008). A high‐affinity human monoclonal antibody specific to the alternatively spliced EDA domain of fibronectin efficiently targets tumor neo‐vasculature in vivo. International journal of cancer, 122(11), 2405-2413.
Specifications
- Immunogen
- Recombinant factor VIII
- Host Species
- Human
- Derivation
- Human phage display scFv library
- Type
- Human IgG
- Specificity
- Human factor VIII
- Species Reactivity
- Human
- Clone
- DH10
- Applications
- WB, IF, ELISA, Dot
- Related Disease
- Hemophilia A
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The F8 antibody has been reported in applications of Western Blot, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Dot blot.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-930-VHH | Recombinant Anti-Human F8 VHH Single Domain Antibody | ELISA, Neut, ICC, FC | Llama VHH |
Rat Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-259CL | Human Anti-F8 Recombinant Antibody (TAB-259CL) | ELISA | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-051 | Mouse Anti-F8 Recombinant Antibody (clone 3E6); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| PFBL-052 | Mouse Anti-F8 Recombinant Antibody (clone G99); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| HPAB-1977-FY-F(E) | Human Anti-F8 Recombinant Antibody (clone 92-137); Fab Fragment | Inhib | Human Fab |
| HPAB-1978-FY-F(E) | Human Anti-F8 Recombinant Antibody (clone 92-102); Fab Fragment | Inhib | Human Fab |
| HPAB-1979-FY-F(E) | Human Anti-F8 Recombinant Antibody (clone WR8); Fab Fragment | IP | Human Fab |
Mouse Antibody
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-014LC | Recombinant Anti-Human F8 Antibody (Fab glycosylation) | ELISA | Human antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-015LC | Recombinant Anti-Human F8 Antibody (Non-glycosylated) | ELISA | Human antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-058 | Recombinant Human Anti-F8 Antibody (BO2C11) | WB, ELISA, FuncS, Inhib, PK, IP, SPR | IgG |
| PABX-058-S (P) | Recombinant Human Anti-F8 Antibody scFv Fragment (BO2C11) | WB, ELISA, FuncS | scFv |
| PABX-058-F (E) | Recombinant Human Anti-F8 Antibody Fab Fragment (BO2C11) | WB, ELISA, FuncS | Fab |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-765CQ | Mouse Anti-F8 Recombinant Antibody (clone CBL215) | ELISA, WB, Neut | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1229 | Hi-Affi™ Rabbit Anti-F8 Recombinant Antibody (clone DS1229AB) | ELISA | Rabbit IgG |
| MOR-4166 | Hi-Affi™ Rabbit Anti-F8 Recombinant Antibody (clone SI243DS) | ELISA | Rabbit IgG |
| MOR-4167 | Hi-Affi™ Rabbit Anti-F8 Recombinant Antibody (clone SI244DS) | ELISA | Rabbit IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0611LC | Human Anti-F8 Recombinant Antibody (clone BO2C11) | ELISA | Human IgG4 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0197-CN-S(P) | Human Anti-F8 Recombinant Antibody (clone KM33); scFv Fragment | WB | Human scFv |
| HPAB-0178CQ-S(P) | Mouse Anti-F8 Recombinant Antibody; scFv Fragment (HPAB-0178CQ-S(P)) | ELISA, DB, Inhib | Mouse scFv |
| FAMAB-0039JF-S(P) | Human Anti-F8 Recombinant Antibody (clone JM4); scFv Fragment | IP, ELISA, FuncS | Human scFv |
| FAMAB-0200JF-S(P) | Human Anti-F8 Recombinant Antibody (clone KM41); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| FAMAB-0201JF-S(P) | Human Anti-F8 Recombinant Antibody (clone KM38); scFv Fragment | WB, ELISA, FuncS | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0178CQ | Mouse Anti-F8 Recombinant Antibody (HPAB-0178CQ) | ELISA, DB, Inhib | Mouse IgG2b, κ |
| HPAB-1242WJ | Human Anti-F8 Recombinant Antibody (clone YK3.3.40) | Inhib | Human IgG |
| HPAB-1243WJ | Human Anti-F8 Recombinant Antibody (clone YK3.3.50) | Inhib | Human IgG |
| FAMAB-0286JF | Human Anti-F8 Recombinant Antibody (clone VK34) | ELISA, FuncS | Human IgG |
| FAMAB-0287JF | Human Anti-F8 Recombinant Antibody (clone VK41) | ELISA, FuncS | Human IgG |
MHC Tetramer for Other Applications
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF075 | DRB1*11:01/Factor VIII Factor VIII (ENIQRFLPNPAGVQLEDPEF) MHC Monomer | MHC Multimer |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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