Rabbit Anti-FGFR3 Recombinant Antibody (clone R03-2C7) (CAT#: VS3-FY2003)

• Immunogen
• Synthetic peptide of human FGFR3

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat FGFR3

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R03-2C7

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 88 kDa, while the observed molecular weight stands at 125 kDa.

• Related Disease
• Achondroplasia and Hypochondroplasia

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Fibroblast Growth Factor Receptor 3; EC 2.7.10.1; FGFR-3; JTK4; Fibroblast Growth Factor Receptor 3 Variant 4; Achondroplasia, Thanatophoric Dwarfism; Hydroxyaryl-Protein Kinase; Tyrosine Kinase JTK4

• Gene ID
• 2261

• UniProt ID
• P22607

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.

• Cellular Localization
• Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Secreted

• Post Translation Modifications
• Autophosphorylated.
  Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues.
  Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer.
  Phosphorylation at Tyr-724 is essential for stimulation of cell proliferation and activation of PIK3R1, STAT1 and MAP kinase signaling.
  Phosphorylation at Tyr-760 is required for interaction with PIK3R1 and PLCG1.
  Ubiquitinated.
  Is rapidly ubiquitinated after ligand binding and autophosphorylation, leading to receptor internalization and degradation.
  Subject to both proteasomal and lysosomal degradation.
  N-glycosylated in the endoplasmic reticulum.
  The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus.
  Glycosylation at Asn98, Asn225, Asn262, Asn294, Asn315, and Asn328 (NX_P22607 [NX_P22607-1])
  Modification sites at PhosphoSitePlus (P22607)
  Glycosylation from GlyGen (P22607) 7 sites, 1 O-linked glycan (1 site)

• Protein Refseq
• XP_006713931.1; XP_006713932.1; XP_006713933.1; XP_006713934.1; XP_006713936.1

• Function
• Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody