Human Anti-ICOS Recombinant Antibody (clone JMab-136); scFv Fragment

Name: Human Anti-ICOS Recombinant Antibody (clone JMab-136); scFv Fragment
SKU: HPAB-0052-YJ-S(P)
Rating: 5 (1 reviews)

CAT#: HPAB-0052-YJ-S(P)

This product is a recombinant human scFv that recognizes human AILIM. The antibody AIH5D3 (JMab-136) is cross-reactive with human AILIM, Mouse AILIM, and Rat AILIM. It is capable of controlling a variety of biological reactions (for example, cell proliferation, cytokine production, immune cytolysis, cell death, induction of ADCC, etc.) associated with AILIM-mediated costimulatory signal (secondary signal) transduction. Furthermore, it has been revealed that AIH5D3 (JMab-136) is effective to treat and prevent various diseases associated with AILIM-mediated costimulatory signal transduction, being capable of inhibiting the onset and/or advancement of the diseases.

Gene Expression Tested Data

Subcellular Location Figure 1 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/34865/1813_D4_33_selected.jpg

Subcellular Location Figure 2 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of lymphoid cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32575/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/34865/1886_G11_2_blue_red_green.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32575/78503_A_9_8.jpg

Normal Tissue Figure 5 Tonsil

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32575/78503_A_6_8.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (HEL, MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163600-ICOS

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SDS-PAGE

Figure 1 Human Anti-ICOS Recombinant Antibody (clone JMab-136); scFv Fragment, gggc tag (HPAB-0052-YJ-S(P)) in SDS-PAGE

Lane 1: Non-reduced scFv
Lane 2: Reduced scFv

Figure 2 Human Anti-ICOS Recombinant Antibody (clone JMab-136); scFv Fragment, gggc tag (HPAB-0052-YJ-S(P)) in WB

Western blot analysis of HPAB-0052-YJ-S(P) was performed by loading recombinant Human ICOS with hFc-tag.

HPAB-0052-YJ-S(P) incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-his tag antibody
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 3 Human Anti-ICOS Recombinant Antibody (clone JMab-136); scFv Fragment, gggc tag (HPAB-0052-YJ-S(P)) in ELISA

ELISA analysis of HPAB-0052-YJ-S(P) was performed by coating with recombinant Human ICOS with hFc-tag.

The secondary antibody: HRP-Anti-his tag antibody

Figure 4 Human Anti-ICOS Recombinant Antibody (clone JMab-136); scFv Fragment, gggc tag (HPAB-0052-YJ-S(P)) in DB

Dot Blot analysis of HPAB-0052-YJ-S(P) was performed by coating with recombinant Human ICOS with hFc-tag.

The secondary antibody: HRP-Anti-his tag antibody

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Specifications

Immunogen
Cell membrane fraction prepared from recombinant cells (HPB-ALL cells or CHO cells) overexpressing human AILIM.

Host Species
Human

Type
Human scFv

Specificity
Human

Species Reactivity
Human, Mouse, Rat

Clone
JMab-136

Applications
ELISA

Related Disease
Disorders related to the delayed allergy

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
AILIM; CD278; CVID1; activation-inducible lymphocyte immunomediatory molecule; inducible T-cell co-stimulator; inducible costimulator; AIH5D3 (JMab-136)

Gene ID
29851

UniProt ID
Q9Y6W8

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0052-YJ-S(P).pdf

MSDS

MSDS - HPAB-0052-YJ-S(P).pdf

COA

Certificate of Analysis Lookup

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