Mouse Anti-KIT Monoclonal Antibody (VS7-0425-WR222)

Name: Mouse Anti-KIT Monoclonal Antibody (VS7-0425-WR222)
SKU: VS7-0425-WR222
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR222

This product is a mouse IgG antibody that binds to human KIT. The antibody can be used for WB, IHC-P, IF, and ELISA assays.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEK 293

Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73252/1469_D7_6_selected.jpg

Normal Tissue Figure 2 High expression in breast

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4471/ihc_selected.jpg

Normal Tissue Figure 3 High expression in lung.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_A_1_4_val_selected.jpg

Normal Tissue Figure 4 Skin

Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_B_8_1.jpg

RNA Expression Figure 5 RNA cell line category: Group enriched (HEL, HMC-1)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157404-KIT

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Specifications

Immunogen
Recombinant human KIT fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG

Specificity
Human KIT

Species Reactivity
Human

Molecule Class
Receptor Tyrosine Kinas

Applications
WB, IHC-P, IF, ELISA

Conjugate
Unconjugated

Related Disease
Piebald trait (PBT); Gastrointestinal stromal tumor (GIST); Testicular germ cell tumor (TGCT); Leukemia, acute myelogenous (AML); Mastocytosis, cutaneous (MASTC); Mastocytosis, systemic (MASTSYS)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
1 mg/ml

Preservative
0.03% sodium azide

Storage
Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-onco

Gene ID
3815

UniProt ID
A0A024RDA0

Long Name
KIT Proto-Oncogene Receptor Tyrosine Kinase

Cellular Localization
Cell membrane; Cytoplasm; Membrane

Post Translation Modifications
Disulfide bond; Glycoprotein; Phosphoprotein; Ubl conjugation

Protein Refseq
NP_000213.1

Function
Kinase; Receptor; Transferase; Tyrosine-protein kinase

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR222.pdf

MSDS

MSDS - VS7-0425-WR222.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

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