Rabbit Anti-MYC (pS62) Recombinant Antibody (clone R09-1E2) (CAT#: VS3-WK2117)
This product is a rabbit recombinant antibody that is specific to phosphorylated MYC. The target of this antibody is phospho-MYC (Ser62). The clone R09-1E2 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, IP analysis at a dilution of 1/20, and IHC staining at a dilution of 1/50-1/100. The molecular weight of MYC (pSer62) observed in WB experiments using this antibody is 57 kDa.
| Sub Cat | Clone | Species Reactivity | Applications | Application Notes | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS3-WK2295 | R09-1E2 | Human, Mouse, Rat | WB, IHC-P, ICC/IF, IP |
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
||||||
| VS3-WK2243 | Mouse, Rat | WB, ICC, IP |
IP: 1/20 WB: 1/500-1/1000 ICC: 1/50-1/200 |
More Infomation
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/if_selected.jpg
Figure 2 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66556/1279_E11_3_selected.jpg
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66556/1279_E11_3_selected.jpg
Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/ihc_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/84/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/84/ihc_selected.jpg
Figure 5 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows distinct nucleolar positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/ihc_selected.jpg
(Immunohistochemical staining of human caudate shows distinct nucleolar positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/ihc_selected.jpg
Figure 6 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/883_H10_1_red_green.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/883_H10_1_red_green.jpg
Figure 7 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/879_G9_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/879_G9_1_red_green.jpg
Figure 8 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_7_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_7_5.jpg
Figure 9 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_4.jpg
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_4.jpg
Figure 10 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_7_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_7_5.jpg
Figure 11 Cervix
(Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_8_3.jpg
(Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_8_3.jpg
Figure 12 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_8.jpg
Figure 13 RNA cell line category: Cell line enhanced (RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136997-MYC
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136997-MYC
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser62 of human, mouse and rat MYC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- MYC Proto-Oncogene, BHLH Transcription Factor; V-Myc Avian Myelocytomatosis Viral Oncogene Homolog; Class E Basic Helix-Loop-Helix Protein 39; Transcription Factor P64; Proto-Oncogene C-Myc; BHLHe39; Myc-Related Translation/Localization Regulatory Factor
- Gene ID
- 4609
- UniProt ID
- P01106
- Long Name
- MYC Proto-Oncogene, BHLH Transcription Factor
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
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Breast Cancer
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Gastric Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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