Rabbit Anti-Phospho-SNCA (S129) Recombinant Antibody (clone 4G3) (CAT#: ZG-0572U)
Rabbit Anti-Phospho-SNCA (S129) Antibody (clone 4G3) displayed high specificity against the SNCA protein of Human.
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Custom Production
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IHC
Figure 1 Rabbit Anti-Phospho-SNCA (S129) Antibody (ZG-0572U) in IHC
IHC image of ZG-0572U diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 2 Rabbit Anti-Phospho-SNCA (S129) Antibody (ZG-0572U) in IF
Immunofluorescence staining of Hela cells(treated with 100mM EGF for 20min) with ZG-0572U at 1:206, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-SNCA (S129)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-SNCA (S129)
- Species Reactivity
- Human
- Clone
- 4G3
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Dementia, Lewy Body and Parkinson Disease 1, Autosomal Dominant.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; NACP; PARK1
- Gene ID
- 6622
- UniProt ID
- P37840
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B031(A) | Recombinant Anti-human SNCA Intrabody [(D-Arg)9] | FC, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B031(G) | Recombinant Anti-human SNCA Intrabody [+36 GFP] | CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B031(T) | Recombinant Anti-human SNCA Intrabody [Tat] | ELISA, IF, Neut, FuncS | scFv-Tat |
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For Research Use Only. Not For Clinical Use.
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