SmartAb™ Recombinant Anti-TGFB1 pH-dependent Antibody (VS-0825-YC326)

Name: SmartAb™ Recombinant Anti-TGFB1 pH-dependent Antibody (VS-0825-YC326)
SKU: VS-0825-YC326
Rating: 5 (1 reviews)

CAT#: VS-0825-YC326

The anti-TGFB1 pH-dependent antibody (VS-0825-YC326) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human TGFB1. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for TGFB1 under acidic conditions compared to physiological pH. Using a yeast surface display system with a pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from TGFB1 at pH 6.0. This pH-sensitive antibody retains its binding affinity on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. Human TGFB1 (Transforming Growth Factor Beta 1) is a specific isoform of the TGF-β cytokine family. It is a key regulator of immune responses, wound healing, and tissue fibrosis.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73356/1901_C17_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/361/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73356/1812_G3_5_cr5a09c41109818_blue_red_green.jpg

Normal Tissue Figure 4 Bone marrow

Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/361/2675_B_6_4.jpg

RNA Expression Figure 5 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105329-TGFB1

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Specifications

Host Species
Human

Type
Human IgG4 kappa

Specificity
Human TGFB1

Species Reactivity
Human

Applications
FC, IP, ELISA, Neut, IF, ICC

Product Property

Clonality
Monoclonal

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE and SEC-HPLC

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.

Target

Alternative Names
TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB;

Gene ID
7040

UniProt ID
P01137

Long Name
Transforming Growth Factor Beta 1

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Related Diseases

Inflammatory Bowel Diseases

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0825-YC326.pdf

MSDS

MSDS - VS-0825-YC326.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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