Rabbit Anti-TP53 Recombinant Antibody (clone 3D4) (CAT#: ZG-0603U)
Rabbit Anti-TP53 Antibody (clone 3D4) displayed high specificity against the TP53 protein of Human.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-TP53 Antibody (ZG-0603U) in WB
Western Blot
Positive WB detected in: COLO320 whole cell lysate, 293 whole cell lysate
All lanes: TP53 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 53 kDa
IHC
Figure 2 Rabbit Anti-TP53 Antibody (ZG-0603U) in IHC
IHC image of ZG-0603U diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-TP53 Antibody (ZG-0603U) in IF
Immunofluorescence staining of HepG2 Cells with ZG-0603U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human p53
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TP53
- Species Reactivity
- Human
- Clone
- 3D4
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Li-Fraumeni Syndrome and Papilloma Of Choroid Plexus.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53
- Gene ID
- 7157
- UniProt ID
- P04637
Related Resources
EGFR Tyrosine Kinase Inhibitor Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-072 | Recombinant Anti-p53 VHH Single Domain Antibody (PNBL-072) | Inhib, IP, WB, ELISA, FuncS | Llama VHH |
| HPAB-0746-YJ-VHH | Camelid Anti-TP53 Recombinant Single Domain Antibody (HPAB-0746-YJ-VHH) | ELISA | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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