CytoStream™ Mouse Anti-CD154 Recombinant Antibody (MMC-M1268)
CAT#: VS-0126-XU356
CytoStream™ Mouse Anti-CD154 Recombinant Antibody (MMC-M1268) is a specialized tool engineered for the high-sensitivity detection of the human CD40 ligand. Utilizing recombinant expression, this antibody ensures high specificity and superior signal-to-noise ratios in flow cytometry (FC). It facilitates the precise characterization of activated T-lymphocyte subsets and provides stable identification across various activation states. The recombinant manufacturing process guarantees exceptional lot-to-lot consistency, offering researchers a robust instrument for exploring the regulatory roles of the TNF-receptor superfamily ligands.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2010_G3_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/2069_B6_2_blue_green.jpg
Normal Tissue
Figure 3 Colon
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_3.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72480/169295_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102245-CD40LG
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| Sub Cat | Product Name | Size | Formulation | Conjugate Group | Characteristics | Stability | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0126-XU356-1 | CytoStream™ Mouse Anti-CD154 Recombinant Antibody (MMC-M1268), APC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | APC | Protein-based fluorochrome with excitation/emission at ~650/660 nm. Exhibits minimal spectral overlap with FITC and PE, ideal for multicolor panels. High brightness. | Sensitive to light and heat. Store at 4°C in the dark. Avoid repeated freeze-thaw cycles. Stable for ≥1 year under proper storage. | ||||||
| VS-0126-XU356-2 | CytoStream™ Mouse Anti-CD154 Recombinant Antibody (MMC-M1268), FITC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | FITC | Small organic fluorophore (Ex/Em: 488/519 nm). High initial brightness but susceptible to photobleaching. Requires careful compensation with PE channel. | Sensitive to light and pH. Prone to rapid photobleaching during acquisition. Store at 4°C in the dark. Prepare working solutions fresh. | ||||||
| VS-0126-XU356-3 | CytoStream™ Mouse Anti-CD154 Recombinant Antibody (MMC-M1268), PE-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE | Phycobiliprotein fluorochrome (Ex/Em peak: 565/575 nm). One of the brightest fluorophores available, ideal for low-abundance antigen detection. Broad emission requires careful compensation. | Moderate stability. Sensitive to light, heat, and harsh fixatives. Store at 4°C in the dark. Avoid freeze-thaw cycles to maintain performance. | ||||||
| VS-0126-XU356-4 | CytoStream™ Mouse Anti-CD154 Recombinant Antibody (MMC-M1268), PE-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy7 | Tandem dye (PE excited, Cy7 emits at ~785 nm). Critical for deep-red infrared detection. Essential: Use a Cy7 (780/60 nm) detector, not a PE filter. Susceptible to tandem bond degradation. | Low stability. Highly sensitive to fixation, light exposure, and storage time. Protect from light and analyze immediately post-fixation. | ||||||
| VS-0126-XU356-5 | CytoStream™ Mouse Anti-CD154 Recombinant Antibody (MMC-M1268), AF Dye (488nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (488nm) | Synthetic green fluorogenic dye (Ex/Em ~490/520 nm). A photostable alternative to FITC with brighter signal and narrower emission spectrum, reducing spillover into PE channel. | Excellent photostability. Maintains signal integrity during prolonged acquisition. Tolerates fixation well and exhibits minimal pH sensitivity. | ||||||
| VS-0126-XU356-6 | CytoStream™ Mouse Anti-CD154 Recombinant Antibody (MMC-M1268), AF Dye (647nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (647nm) | Synthetic far-red fluorogenic dye (Ex/Em ~650/665 nm). Spectrally similar to APC but with enhanced brightness and photostability. Ideal for replacing APC in demanding protocols. | Superior stability. Highly resistant to photobleaching and pH fluctuations. Compatible with harsh fixation and permeabilization protocols. | ||||||
| VS-0126-XU356-7 | CytoStream™ Purified Mouse Anti-CD154 Recombinant Antibody (MMC-M1268) | 100 ug | None | The native antibody without any fluorophore. Used as a primary antibody in indirect staining or for functional assays (blocking, neutralization). Requires a compatible fluorescent secondary antibody for detection. | Typically exhibits the highest intrinsic stability among all formats. Storage conditions are defined by the antibody's own formulation buffer. |
More Infomation
Specifications
- Host Species
- Mouse
- Type
- Monoclonal Antibody
- Specificity
- CD154
- Species Reactivity
- Human
- Applications
- FC
Product Property
- Clonality
- Monoclonal
- Storage
- Store at 4˚C.
- Shipping
- Ice Packs
Applications
- Application Notes
- Test: 5 μl/Test
Notice: This reagent is supplied in a pre-optimized, ready-to-use formulation for direct application at the specified volume per test in flow cytometry. The standard protocol is suitable for staining up to 1 million cells per assay. Optimal performance may require protocol adjustments based on specific experimental conditions.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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