Recombinant Rabbit Anti-APC Antibody (clone R06-2E4) (CAT#: VS3-FY82)
This product is a recombinant rabbit antibody that recognizes human APC protein. It can detect a band of approximately 312 kDa (observedd molecular weight: 160 kDa).
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ICC
Figure 1. Recombinant Rabbit Anti-APC Antibody (clone R06-2E4) in ICC.
Immunocytochemical analysis of APC (green) in LNcap using APC antibody and DAPI (blue).
ICC
Figure 2. Recombinant Rabbit Anti-APC Antibody (clone R06-2E4) in ICC.
Immunocytochemical analysis of APC (green) in LNcap using APC antibody and DAPI (blue).
Specifications
- Immunogen
- A synthetic peptide of human APC
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human APC
- Species Reactivity
- Human
- Clone
- R06-2E4
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 312 kDa; Observed MW: 160 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- APC, WNT Signaling Pathway Regulator; Protein Phosphatase 1, Regulatory Subunit 46; Deleted In Polyposis 2.5; DP2.5; Adenomatosis Polyposis Coli Tumor Suppressor; Truncated Adenomatosis Polyposis Coli; Adenomatous Polyposis Coli Protein; WNT Signaling Pathway Regulator; Adenomatosis Polyposis Coli
- Gene ID
- 324
- UniProt ID
- P25054
- Sequence Similarities
- Belongs to the adenomatous polyposis coli (APC) family.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule
- Post Translation Modifications
- Phosphorylated by GSK3B.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
- Protein Refseq
- NP_000029.2; NP_001120982.1; NP_001120983.2
- Function
- Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Cushing Syndrome
Canonical Wnt Signaling Pathway
JAK-STAT Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "APC"
Select a product category from the dropdown menu below to view related products.
Please select product type
Fab Antibody Products
ScFv Antibody Products
Human Antibody Products
IgG Antibody Products
Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-72-F(E) | Recombinant Anti-human APC Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| HPAB-J0036-YC-F(E) | Mouse Anti-aPC Recombinant Antibody; Fab Fragment (HPAB-J0036-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-J0037-YC-F(E) | Mouse Anti-aPC Recombinant Antibody; Fab Fragment (HPAB-J0037-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-J0038-YC-F(E) | Mouse Anti-aPC Recombinant Antibody; Fab Fragment (HPAB-J0038-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-J0039-YC-F(E) | Mouse Anti-aPC Recombinant Antibody; Fab Fragment (HPAB-J0039-YC-F(E)) | ELISA | Mouse Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-FY82. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
-2.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, FC, WB, Block
Application: ELISA, FC
Application: FC, ADCC, CDC, Inhib
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, IHC, WB
-2.png)
Application: WB, ELISA, FuncS
Application: ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
